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验证数据展示

  • FC experiment of human PBMCs using SF98063-1-RR (same clone as SF98063-1-PBS)

    FC experiment of human PBMCs using SF98063-1-RR (same clone as SF98063-1-PBS)

    1x10^6 untreated or PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human LIGHT/CD258 Rabbit scFv Antibody (SF98063-1-RR, Clone: 240694D7) and PE Anti-6*His, His-Tag Rabbit RecAb (PE-FcA98450, Clone: 251107A1). Cells were then stained with CL647 Anti-Human CD8. Cells were not fixed. This data was developed using the same antibody clone with SF98063-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using SF98063-1-RR (same clone as SF98063-1-PBS)

    FC experiment of human PBMCs using SF98063-1-RR (same clone as SF98063-1-PBS)

    1x10^6 PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug anti-human LIGHT/CD258 Recombinant Antibody (SF98063-1-RR, Clone: 240694D7) (red) or 0.25 ug Rabbit IgG Isotype Control Rabbit scFv Antibody (SF98136-1-RR, Clone: 240953C9) (blue), and PE Anti-6*His, His-Tag Rabbit RecAb (PE-FcA98450, Clone: 251107A1). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug anti-human LIGHT/CD258 Recombinant Antibody (SF98063-1-RR, Clone: 240694D7) (black, dashed) and PE Anti-6*His, His-Tag Rabbit RecAb (PE-FcA98450, Clone: 251107A1). Cells were not fixed. This data was developed using the same antibody clone with SF98063-1-PBS in a different storage buffer formulation.

  • Affinity of SF98063-1-RR

    Affinity of SF98063-1-RR

    Biolayer interferometry (BLl) kinetic assays of SF98063-1-RR against Human LIGHT/CD258 were performed. The affinity constant is 1.72 nM.

Anti-Human LIGHT/CD258 Rabbit scFv Antibody, PBS Only

LIGHT/CD258 Recombinant Antibody for FC, Indirect ELISA
Cat No. SF98063-1-PBS

产品说明书

CloneNo. 240694D7

宿主/类型

Rabbit / scFv

种属反应性

human

应用

FC, Indirect ELISA

别名

LIGHT, CD258, HVEM L, HVEML, HVEM-L

缓冲液配方: 
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy TNFSF14/LIGHT Ready-To-Use IHC Kit. TNFSF14/LIGHT primary antibody included.

产品信息

SF98063-1-PBS targets LIGHT/CD258 in FC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 FC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg32095

Product name: Recombinant Human LIGHT/CD258 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec

Tag: N-6*his

Domain: 74-240 aa of NM_003807

Sequence: DGPAGSWEQLIQERRSHEVNPAAHLTGANSSLTGSGGPLLWETQLGLAFLRGLSYHDGALVVTKAGYYYIYSKVQLGGVGCPLGLASTITHGLYKRTPRYPEELELLVSQQSPCGRATSSSRVWWDSSFLGGVVHLEAGEKVVVRVLDERLVRLRDGTRSYFGAFMV

种属同源性预测
宿主/类型 Rabbit / scFv
抗体类别 Recombinant
产品类型 Single-chain variable fragment antibody (scFv)
全称 tumor necrosis factor (ligand) superfamily, member 14
别名 LIGHT, CD258, HVEM L, HVEML, HVEM-L
计算分子量26KD
GenBank蛋白编号NM_003807
基因名称 LIGHT
Gene ID (NCBI) 8740
RRIDAB_3750146
偶联类型 Unconjugated
形式Liquid
纯化方式Affinity purification
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LIGHT, also known as TNFSF14, HVEML, or CD258, is a type II transmembrane protein that belongs to the TNF superfamily (PMID: 9462508). It is predominantly expressed in lymphoid tissues and produced by activated T cells and immature dendritic cells (DCs) (PMID: 9462508; 10754304). Through its two primary functional receptors HVEM (TNFRSF14) and lymphotoxin β receptor (LTβR), LIGHT signaling is involved in development and maintenance of lymphoid tissues, as well as innate and adaptive immune responses (PMID: 24575096; 26720335). In addition to the membrane form, LIGHT can also exist as a soluble form generated by cleavage of the extracellular portion of the membrane form.

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