验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293T cells, HeLa cells, PC-3 cells, HepG2 cells |
| Positive IP detected in | HEK-293T cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26583-1-AP targets DNA ligase III/LIG3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 |
CatNo: Ag24998 Product name: Recombinant human LIG3 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 254-390 aa of BC068005 Sequence: CDPRHKDCLLREFRKLCAMVADNPSYNTKTQIIQDFLRKGSAGDGFHGDVYLTVKLLLPGVIKTVYNLNDKQIVKLFSRIFNCNPDDMARDLEQGDVSETIRVFFEQSKSFPPAAKSLLTIQEVDEFLLRLSKLTKE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ligase III, DNA, ATP-dependent |
| 别名 | LIG3, DNA ligase 3, DNA ligase III, EC:6.5.1.1, LIG2 |
| 计算分子量 | 100-110 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC068005 |
| 基因名称 | LIG3 |
| Gene ID (NCBI) | 3980 |
| RRID | AB_2880562 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P49916 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DNA ligase III(DNA ligase 3) is an enzyme that in humans is encoded by the LIG3 gene. The human LIG3 gene encodes ATP-dependent DNA ligases that seal interruptions in the phosphodiester backbone of duplex DNA. There are three families of ATP-dependent DNA ligases in eukaryotes. These enzymes utilize the same three step reaction mechanism; 1 formation of a covalent enzyme-adenylate intermediate; 2 transfer of the adenylate group to the 5' phosphate terminus of a DNA nick; 3 phosphodiester bond formation. Unlike LIG1 and LIG4 family members that are found in almost all eukaryotes, LIG3 family members are less widely distributed. The LIG3 gene encodes several distinct DNA ligase species by alternative translation initiation and alternative splicing mechanisms.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for DNA ligase III/LIG3 antibody 26583-1-AP | Download protocol |
| IHC protocol for DNA ligase III/LIG3 antibody 26583-1-AP | Download protocol |
| IP protocol for DNA ligase III/LIG3 antibody 26583-1-AP | Download protocol |
| WB protocol for DNA ligase III/LIG3 antibody 26583-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature Retrotransposons hijack alt-EJ for DNA replication and eccDNA biogenesis
| ||
Mol Cell Microhomology-mediated end joining acts directly on replication forks to repair single-ended double-strand breaks. | ||
Brain Biallelic variants in LIG3 cause a novel mitochondrial neurogastrointestinal encephalomyopathy. | ||
Clin Transl Med Extrachromosomal circular DNA expressing miRNA promotes ovarian cancer progression.
| ||
J Cell Mol Med Berberine Sensitises Breast Cancer Cells to Radiation via the Attenuation of DNA Ligase III |
