LIG3 Polyclonal antibody
LIG3 Polyclonal Antibody for IF/ICC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
IF/ICC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293T cells, HeLa cells, PC-3 cells |
Positive IP detected in | HEK-293T cells |
Positive IF detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
26583-1-AP targets LIG3 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | IF/ICC, IP, WB, ELISA |
Cited Applications | WB, IF |
Tested Reactivity | human, mouse |
Cited Reactivity | human, pig |
Immunogen | LIG3 fusion protein Ag24998 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ligase III, DNA, ATP-dependent |
Synonyms | DNA ligase 3, DNA ligase III, LIG2, LIG3, ligase III, DNA, ATP dependent |
Calculated Molecular Weight | 100-110 kDa |
Observed Molecular Weight | 100 kDa |
GenBank Accession Number | BC068005 |
Gene Symbol | LIG3 |
Gene ID (NCBI) | 3980 |
RRID | AB_2880562 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P49916 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for LIG3 antibody 26583-1-AP | Download protocol |
IF protocol for LIG3 antibody 26583-1-AP | Download protocol |
IP protocol for LIG3 antibody 26583-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Brain Biallelic variants in LIG3 cause a novel mitochondrial neurogastrointestinal encephalomyopathy. | ||
Int J Biochem Cell Biol Increasing CRISPR/Cas9-mediated homology-directed DNA repair by histone deacetylase inhibitors. | ||
bioRxiv D-type cyclins regulate DNA mismatch repair in the G1 and S phases of the cell cycle, maintaining genome stability | ||
Nature Retrotransposons hijack alt-EJ for DNA replication and eccDNA biogenesis
|