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验证数据展示

  • WB analysis using 30722-1-AP

    WB analysis using 30722-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30722-1-AP (LHX8 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 30722-1-AP

    IF Staining of A549 using 30722-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using LHX8 antibody (30722-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

LHX8 Polyclonal antibody

LHX8 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30722-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

LIM homeobox protein 8, LIM homeobox 8, Lhx7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30722-1-AP targets LHX8 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 LHX8 fusion protein Ag33787 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 LIM homeobox 8
别名 LIM homeobox protein 8, LIM homeobox 8, Lhx7
计算分子量 39 kDa
观测分子量39-44 kDa
GenBank蛋白编号BC040321
基因名称 LHX8
Gene ID (NCBI) 431707
RRIDAB_3086401
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ68G74
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LHX8 (LIM homeobox 8), also known as LHX7. It is expected to be located in nucleus and mainly expressed in brain, lymphoid tissue, salivary gland. The calculated molecular weight of METAP1 is 39 kDa. The protein encoded by this gene is a member of the LIM homeobox family of proteins, which are involved in patterning and differentiation of various tissue types. These proteins contain two tandemly repeated cysteine-rich double-zinc finger motifs known as LIM domains, in addition to a DNA-binding homeodomain. It is also involved in oogenesis and in neuronal differentiation. LHX8 is a candidate gene for cleft palate, and it is also associated with odontoma formation (PMID: 34112263).

实验方案

Product Specific Protocols
WB protocol for LHX8 antibody 30722-1-APDownload protocol
IF protocol for LHX8 antibody 30722-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols