验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, rat brain tissue |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 1 publications below |
IF | See 2 publications below |
产品信息
30007-1-AP targets LGR5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, ELISA Application Description |
文献引用应用 | IHC, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | mouse |
免疫原 | LGR5 fusion protein Ag31625 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | leucine-rich repeat-containing G protein-coupled receptor 5 |
别名 | G-protein coupled receptor HG38, G-protein coupled receptor 67, G-protein coupled receptor 49, GPR67, GPR49 |
计算分子量 | 907 aa, 100 kDa |
观测分子量 | 100 kDa |
GenBank蛋白编号 | BC096324 |
基因名称 | LGR5 |
Gene ID (NCBI) | 8549 |
RRID | AB_3086207 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O75473 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5) also known as G-protein coupled receptor 49 (GPR49) or G-protein coupled receptor 67 (GPR67) is a protein that in humans is encoded by the LGR5 gene. It is a member of GPCR class A receptor proteins. R-spondin proteins are the biological ligands of LGR5. LGR5 is expressed across a diverse range of tissue such as in the muscle, placenta, spinal cord and brain and particularly as a biomarker of adult stem cells in certain tissues.LGR5 is crucial during embryogenesis as LGR null studies in mice incurred 100% neonatal mortality accompanied by several craniofacial distortions such as ankyloglossia and gastrointestinal dilation (PMID: 9642114, 16575208, 525477).
实验方案
Product Specific Protocols | |
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WB protocol for LGR5 antibody 30007-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Burns Trauma Nocardia rubra cell-wall skeleton mitigates whole abdominal irradiation-induced intestinal injury via regulating macrophage function | ||
Acta Histochem Stimulation of mouse hair regrowth by exosomes derived from human umbilical cord mesenchymal stem cells | ||
Zool Res Peptide Cy RL-QN15 accelerates hair regeneration in diabetic mice by binding to the Frizzled-7 receptor |