LGR5 Polyclonal antibody

LGR5 Polyclonal Antibody for WB, ELISA
Cat No. 30007-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA and More (2)

FEX, GPR49, GPR67, G-protein coupled receptor 49, G-protein coupled receptor 67

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inBGC-823 cells, mouse brain tissue, HEK-293 cells, SGC-7901 cells, SKOV-3 cells, rat brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30007-1-AP targets LGR5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
文献引用应用IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原

CatNo: Ag31625

Product name: Recombinant human LGR5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 824-907 aa of BC096324

Sequence: NPHFKEDLVSLRKQTYVWTRSKHPSLMSINSDDVEKQSCDSTQALVTFTSSSITYDLPPSSVPSPAYPVTESCHLSSVAFVPCL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 leucine-rich repeat-containing G protein-coupled receptor 5
别名 FEX, GPR49, GPR67, G-protein coupled receptor 49, G-protein coupled receptor 67
计算分子量 907 aa, 100 kDa
观测分子量100 kDa
GenBank蛋白编号BC096324
基因名称 LGR5
Gene ID (NCBI) 8549
RRIDAB_3086207
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75473
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5) also known as G-protein coupled receptor 49 (GPR49) or G-protein coupled receptor 67 (GPR67) is a protein that in humans is encoded by the LGR5 gene. It is a member of GPCR class A receptor proteins. R-spondin proteins are the biological ligands of LGR5. LGR5 is expressed across a diverse range of tissue such as in the muscle, placenta, spinal cord and brain and particularly as a biomarker of adult stem cells in certain tissues.LGR5 is crucial during embryogenesis as LGR null studies in mice incurred 100% neonatal mortality accompanied by several craniofacial distortions such as ankyloglossia and gastrointestinal dilation (PMID: 9642114, 16575208, 525477).

实验方案

Product Specific Protocols
WB protocol for LGR5 antibody 30007-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Burns Trauma

Nocardia rubra cell-wall skeleton mitigates whole abdominal irradiation-induced intestinal injury via regulating macrophage function

Authors - Lingling Wu
mouseIF

Acta Histochem

Stimulation of mouse hair regrowth by exosomes derived from human umbilical cord mesenchymal stem cells

Authors - Yang Jiao
mouseIF

Zool Res

Peptide Cy RL-QN15 accelerates hair regeneration in diabetic mice by binding to the Frizzled-7 receptor

Authors - Yu-Tong Wu
IF

Int J Biol Macromol

Integrated bulk and single-cell RNA sequencing unveils the temporal and spatial dynamics of epidermal cell adhesion

Authors - Qingbo Zheng
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