LGR4 Polyclonal antibody

LGR4 Polyclonal Antibody for IHC, ELISA
Cat No. 20150-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

IHC, ELISA and More (1)

G protein coupled receptor 48, GPR48, LGR4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20150-1-AP targets LGR4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, pig
免疫原 LGR4 fusion protein Ag13695 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 leucine-rich repeat-containing G protein-coupled receptor 4
别名 G protein coupled receptor 48, GPR48, LGR4
计算分子量 104 kDa
GenBank蛋白编号BC033039
基因名称 LGR4
Gene ID (NCBI) 55366
RRIDAB_2878648
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BXB1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LGR4, also known as GPR48, is a membrane of the leucine-rich GPCR family. LGR4 is composed of a large NH2-terminal extracellular domain containing 18 LRRs, the seven membrane-spanning segments, and a COOH-terminal intracellular domain. LGR4 is a receptor for R-spondins that potentiates the canonical Wnt signaling pathway and is involved in the formation of various organs. It has also been shown that LGR4 regulates cyclic AMP/protein kinase A (PKA) signalling. (PMID: 23756652; 17178856; 24212090)

实验方案

Product Specific Protocols
IHC protocol for LGR4 antibody 20150-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nat Cell Biol

LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis.

Authors - Pingping Zhu
humanWB

Mol Ther Oncolytics

Silencing LGR6 Attenuates Stemness and Chemoresistance via Inhibiting Wnt/β-Catenin Signaling in Ovarian Cancer.

Authors - Xiaohong Ruan
humanIHC

Cancer Biomark

LGR4 overexpression is associated with clinical parameters and poor prognosis of serous ovarian cancer

Authors - Zhaoyang Zeng
humanIHC

Exp Cell Res

Silencing of RSPO1 mitigates obesity-related renal fibrosis in mice by deactivating Wnt/β-catenin pathway.

Authors - Xuesong Su
mouseWB

In Vitro Cell Dev Biol Anim

SOX2 suppresses osteoblast differentiation of MC3T3-E1 cells through activating the transcription of LGR4

Authors - Sunyu Chen
pig,mouseWB

Cell Death Dis

DNA methylation mediated RSPO2 to promote follicular development in mammals.

Authors - Xiaofeng Zhou
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