验证数据展示
经过测试的应用
Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 5 publications below |
IHC | See 2 publications below |
产品信息
20150-1-AP targets LGR4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | IHC, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, pig |
免疫原 | LGR4 fusion protein Ag13695 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | leucine-rich repeat-containing G protein-coupled receptor 4 |
别名 | G protein coupled receptor 48, GPR48, LGR4 |
计算分子量 | 104 kDa |
GenBank蛋白编号 | BC033039 |
基因名称 | LGR4 |
Gene ID (NCBI) | 55366 |
RRID | AB_2878648 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9BXB1 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LGR4, also known as GPR48, is a membrane of the leucine-rich GPCR family. LGR4 is composed of a large NH2-terminal extracellular domain containing 18 LRRs, the seven membrane-spanning segments, and a COOH-terminal intracellular domain. LGR4 is a receptor for R-spondins that potentiates the canonical Wnt signaling pathway and is involved in the formation of various organs. It has also been shown that LGR4 regulates cyclic AMP/protein kinase A (PKA) signalling. (PMID: 23756652; 17178856; 24212090)
实验方案
Product Specific Protocols | |
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IHC protocol for LGR4 antibody 20150-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Cell Biol LncGata6 maintains stemness of intestinal stem cells and promotes intestinal tumorigenesis. | ||
Mol Ther Oncolytics Silencing LGR6 Attenuates Stemness and Chemoresistance via Inhibiting Wnt/β-Catenin Signaling in Ovarian Cancer. | ||
Cancer Biomark LGR4 overexpression is associated with clinical parameters and poor prognosis of serous ovarian cancer | ||
Exp Cell Res Silencing of RSPO1 mitigates obesity-related renal fibrosis in mice by deactivating Wnt/β-catenin pathway. | ||
In Vitro Cell Dev Biol Anim SOX2 suppresses osteoblast differentiation of MC3T3-E1 cells through activating the transcription of LGR4 | ||
Cell Death Dis DNA methylation mediated RSPO2 to promote follicular development in mammals. |