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验证数据展示

  • WB analysis of HT-29 using 66686-1-Ig

    WB analysis of HT-29 using 66686-1-Ig

    HT-29 cells were subjected to SDS PAGE followed by western blot with 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 66686-1-Ig

    IHC staining of human colon using 66686-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (Galectin-4 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LGALS4 antibody included
  • IHC staining of human colon using 66686-1-Ig

    IHC staining of human colon using 66686-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (Galectin-4 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LGALS4 antibody included
  • IF Staining of human colon cancer using 66686-1-Ig

    IF Staining of human colon cancer using 66686-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Galectin-4 antibody (66686-1-Ig, Clone: 3C2G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human colon cancer using 66686-1-Ig

    IF Staining of human colon cancer using 66686-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Galectin-4 antibody (66686-1-Ig, Clone: 3C2G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Galectin-4 Monoclonal antibody

Galectin-4 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 66686-1-Ig

产品说明书

CloneNo. 3C2G3

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF-P, ELISA

别名

3C2G3, Antigen NY CO 27, Antigen NY-CO-27, Gal 4, GAL4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格价格库存

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经过测试的应用

Positive WB detected inHT-29 cells, human ileum tissue
Positive IHC detected inhuman colon tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon cancer tissue
Planning an IHC experiment? We recommend our IHCeasy LGALS4 Ready-To-Use IHC Kit. LGALS4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:2000-1:5000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66686-1-Ig targets Galectin-4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4049

Product name: Recombinant human LGALS4,GAL4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-323 aa of BC034750

Sequence: MAYVPAPGYQPTYNPTLPYYQPIPGGLNVGMSVYIQGVASEHMKRFFVNFVVGQDPGSDVAFHFNPRFDGWDKVVFNTLQGGKWGSEERKRSMPFKKGAAFELVFIVLAEHYKVVVNGNPFYEYGHRLPLQMVTHLQVDGDLQLQSINFIGGQPLRPQGPPMMPPYPGPGHCHQQLNSLPTMEGPPTFNPPVPYFGRLQGGLTARRTIIIKGYVPPTGKSFAINFKVGSSGDIALHINPRMGNGTVVRNSLLNGSWGSEEKKITHNPFGPGQFFDLSIRCGLDRFKVYANGQHLFDFAHRLSAFQRVDTLEIQGDVTLSYVQI

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 lectin, galactoside-binding, soluble, 4
别名 3C2G3, Antigen NY CO 27, Antigen NY-CO-27, Gal 4, GAL4
计算分子量 323 aa, 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC034750
基因名称 Galectin-4
Gene ID (NCBI) 3960
RRIDAB_2882040
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP56470
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-4 contains two arbohydrate recognition domains (CRDs) and is expressed in the epithelium of oral mucosa, esophagus, and intestinal and colonic mucosa (PMID: 15546874; 15115909). Aberrant expression of galectin-4 has been reported in several cancers.

实验方案

Product Specific Protocols
FC protocol for Galectin-4 antibody 66686-1-IgDownload protocol
IF protocol for Galectin-4 antibody 66686-1-IgDownload protocol
IHC protocol for Galectin-4 antibody 66686-1-IgDownload protocol
WB protocol for Galectin-4 antibody 66686-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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