• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Galectin-4 Monoclonal antibody

Galectin-4 Monoclonal Antibody for FC, IF-P, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

FC, IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3C2G3

Cat No : 66686-1-Ig

Print datasheet

Synonyms

Antigen NY CO 27, Gal 4, GAL4, Galectin-4, L 36 lactose binding protein, L36LBP, Lactose binding lectin 4, LGALS4

验证数据展示

  • WB analysis of HT-29 using 66686-1-Ig

    WB analysis of HT-29 using 66686-1-Ig

    HT-29 cells were subjected to SDS PAGE followed by western blot with 66686-1-Ig (LGALS4,GAL4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 66686-1-Ig

    IHC staining of human colon using 66686-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (Galectin-4 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LGALS4 antibody included
  • IHC staining of human colon using 66686-1-Ig

    IHC staining of human colon using 66686-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66686-1-Ig (Galectin-4 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with LGALS4 antibody included
  • IF Staining of human colon cancer using 66686-1-Ig

    IF Staining of human colon cancer using 66686-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Galectin-4 antibody (66686-1-Ig, Clone: 3C2G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human colon cancer using 66686-1-Ig

    IF Staining of human colon cancer using 66686-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using Galectin-4 antibody (66686-1-Ig, Clone: 3C2G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of HT-29 using 66686-1-Ig

    FC experiment of HT-29 using 66686-1-Ig

    1X10^6 HT-29 cells were intracellularly stained with 0.4 ug Anti-Human Galectin-4 (66686-1-Ig, Clone:3C2G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHT-29 cells, human ileum tissue
Positive IHC detected inhuman colon tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman colon cancer tissue
Positive FC detected inHT-29 cells
Planning an IHC experiment? We recommend our IHCeasy LGALS4 Ready-To-Use IHC Kit. LGALS4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:2000-1:5000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66686-1-Ig targets Galectin-4 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications FC, IF-P, IHC, WB, ELISA
Tested Reactivity Human
Immunogen Galectin-4 fusion protein Ag4049 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name lectin, galactoside-binding, soluble, 4
Synonyms Antigen NY CO 27, Gal 4, GAL4, Galectin-4, L 36 lactose binding protein, L36LBP, Lactose binding lectin 4, LGALS4
Calculated Molecular Weight 323 aa, 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC034750
Gene Symbol LGALS4
Gene ID (NCBI) 3960
RRIDAB_2882040
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP56470
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Galectins are a family of animal lectins defined by shared characteristic amino-acid sequences and affinity for β-galactose-containing oligosac-charides (PMID: 8063692). Galectin-4 contains two arbohydrate recognition domains (CRDs) and is expressed in the epithelium of oral mucosa, esophagus, and intestinal and colonic mucosa (PMID: 15546874; 15115909). Aberrant expression of galectin-4 has been reported in several cancers.

实验方案

Product Specific Protocols
WB protocol for Galectin-4 antibody 66686-1-IgDownload protocol
IHC protocol for Galectin-4 antibody 66686-1-IgDownload protocol
IF protocol for Galectin-4 antibody 66686-1-IgDownload protocol
FC protocol for Galectin-4 antibody 66686-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols