验证数据展示
经过测试的应用
| Positive WB detected in | mouse small intestine tissue, rat small intestine tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 2 publications below |
产品信息
12849-2-AP targets Galectin 2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag3979 Product name: Recombinant human LGALS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2-130 aa of BC029063 Sequence: ELEVKNMDMKPGSTLKITGSIADGTDGFVINLGQGTDKLNLHFNPRFSESTIVCNSLDGSNWGQEQREDHLCFSPGSEVKFTVTFESDKFKVKLPDGHELTFPNRLGHSHLSYLSVRGGFNMSSFKLKE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lectin, galactoside-binding, soluble, 2 |
| 别名 | Beta-galactoside-binding lectin L-14-II, Gal 2, Gal-2, HL14, Lactose binding lectin 2 |
| 计算分子量 | 132 aa, 15 kDa |
| 观测分子量 | 15 kDa |
| GenBank蛋白编号 | BC029063 |
| 基因名称 | Galectin 2 |
| Gene ID (NCBI) | 3957 |
| RRID | AB_2136753 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P05162 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Galectins are a class of carbohydrate-binding proteins implicated in multiple physiological processes including inflammation, immune responses, cell migration, autophagy, and signal transduction (PMID: 29717004). Galectin-2 (Gal-2, also known as LGALS2) is a monocyte-derived carbohydrate-binding lectin; its upregulation correlates with reduced collateral arteriogenesis in patients with obstructive coronary artery disease (PMID: 25884209). Additionally, Gal-2 may interact with T cells, particularly in the setting of inflammatory bowel disease (PMID: 15356130).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Galectin 2 antibody 12849-2-AP | Download protocol |
| WB protocol for Galectin 2 antibody 12849-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncotarget Design of a peptidic inhibitor that targets the dimer interface of a prototypic galectin. | ||
J Gastroenterol Lowered expression of galectin-2 is associated with lymph node metastasis in gastric cancer. | ||
Oncotarget Galectin signatures contribute to the heterogeneity of breast cancer and provide new prognostic information and therapeutic targets. |
