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验证数据展示

  • WB analysis using 13213-1-AP

    WB analysis using 13213-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13213-1-AP (ARH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 13213-1-AP

    WB analysis of HeLa using 13213-1-AP

    WB result of ARH antibody (13213-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ARH transfected HeLa cells.

  • IHC staining of human liver cancer using 13213-1-AP

    IHC staining of human liver cancer using 13213-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13213-1-AP (ARH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 13213-1-AP

    IHC staining of human liver cancer using 13213-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13213-1-AP (ARH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HeLa using 13213-1-AP

    IP experiment of HeLa using 13213-1-AP

    IP result of anti-ARH (IP:13213-1-AP, 4ug; Detection:13213-1-AP 1:5000) with HeLa cells lysate 1200 ug.

  • IHC staining of mouse kidney using 13213-1-AP

    IHC staining of mouse kidney using 13213-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13213-1-AP (ARH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

ARH Polyclonal antibody

ARH Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 13213-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, ELISA

别名

LDLRAP1, ARH1, ARH2, FHCB1, FHCB2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

13213-1-AP targets ARH in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性mouse
免疫原

CatNo: Ag4006

Product name: Recombinant human LDLRAP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-308 aa of BC029770

Sequence: MDALKSAGRALIRSPSLAKQSWGGGGRHRKLPENWTDTRETLLEGMLFSLKYLGMTLVEQPKGEELSAAAIKRIVATAKASGKKLQKVTLKVSPRGIILTDNLTNQLIENVSIYRISYCTADKMHDKVFAYIAQSQHNQSLECHAFLCTKRKMAQAVTLTVAQAFKVAFEFWQVSKEEKEKRDKASQEGGDVLGARQDCTPPLKSLVATGNLLDLEETAKAPLSTVSANTTNMDEVPRPQALSGSSVVWELDDGLDEAFSRLAQSRTNPQVLDTGLTAQDMHYAQCLSPVDWDKPDSSGTEQDDLFSF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 low density lipoprotein receptor adaptor protein 1
别名 LDLRAP1, ARH1, ARH2, FHCB1, FHCB2
计算分子量 308 aa, 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC029770
基因名称 ARH
Gene ID (NCBI) 26119
RRIDAB_2135283
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5SW96
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ARH antibody 13213-1-APDownload protocol
IHC protocol for ARH antibody 13213-1-APDownload protocol
IP protocol for ARH antibody 13213-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Rep

A multienzyme S-nitrosylation cascade regulates cholesterol homeostasis

Authors - Colin T Stomberski
View Article
mouseWB

Cell Biol Toxicol

Activation of lipophagy ameliorates cadmium-induced neural tube defects via reducing low density lipoprotein cholesterol levels in mouse placentas

Authors - Yu-Feng Zhang
View Article