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LDLR Recombinant antibody

LDLR Recombinant Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1O11

Cat No : 82724-1-RR

Print datasheet

Synonyms

FH, FHC, LDL receptor, LDLR

验证数据展示

  • WB analysis using 82724-1-RR

    WB analysis using 82724-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82724-1-RR (LDLR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 82724-1-RR

    IHC staining of mouse brain using 82724-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82724-1-RR (LDLR antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 82724-1-RR

    IHC staining of mouse brain using 82724-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82724-1-RR (LDLR antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82724-1-RR targets LDLR in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen LDLR fusion protein Ag1236 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name low density lipoprotein receptor
Synonyms FH, FHC, LDL receptor, LDLR
Calculated Molecular Weight 95 kDa
Observed Molecular Weight140-160 kDa
GenBank Accession NumberBC014514
Gene Symbol LDLR
Gene ID (NCBI) 3949
ENSEMBL Gene IDENSG00000130164
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01130
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LDLR (low density lipoprotein receptor) is a member of the LDL receptor gene family and is involved in receptor-mediated endocytosis of specific ligands. The LDLR is a cell surface glycoprotein that scavenges LDL from the blood and regulates plasma LDL cholesterol. The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits, which promotes the rapid endocytosis of bound LDL. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.

实验方案

Product Specific Protocols
WB protocol for LDLR antibody 82724-1-RRDownload protocol
IHC protocol for LDLR antibody 82724-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols