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验证数据展示

  • WB analysis using 14398-1-AP (same clone as 14398-1-PBS)

    WB analysis using 14398-1-AP (same clone as 14398-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14398-1-AP (LDHD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 14398-1-AP (same clone as 14398-1-PBS)

    IHC staining of human liver cancer using 14398-1-AP (same clone as 14398-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14398-1-AP (LDHD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 14398-1-AP (same clone as 14398-1-PBS)

    IHC staining of human liver cancer using 14398-1-AP (same clone as 14398-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14398-1-AP (LDHD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 14398-1-AP (same clone as 14398-1-PBS)

    IF Staining of HepG2 using 14398-1-AP (same clone as 14398-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using LDHD antibody 14398-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 14398-1-PBS in a different storage buffer formulation.

LDHD Polyclonal antibody, PBS Only

LDHD Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14398-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

14398-1-PBS targets LDHD in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5897

Product name: Recombinant human LDHD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 159-507 aa of BC047902

Sequence: GADASLCGMAATGASGTNAVRYGTMRDNVLNLEVVLPDGRLLHTAGRGRHFRFGFWPEIPHHTAWYSPCVSLGRRKSAAGYNLTGLFVGSEGTLGLITATTLRLHPAPEATVAATCAFPSVQAAVDSTVHILQAAVPVARIEFLDEVMMDACNRYSKLNCLVAPTLFLEFHGSQQALEEQLQRTEEIVQQNGASDFSWAKEAEERSRLWTARHNAWYAALATRPGCKGYSTDVCVPISRLPEIVVQTKEDLNASGLTGSIVGHVGDGNFHCILLVNPDDAEELGRVKAFAEQLGRRALALHGTCTGEHGIGMGKRQLLQEEVGAVGVETMRQLKAVLDPQGLMNPGKVL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lactate dehydrogenase D
别名
计算分子量 52 kDa
观测分子量 45-54 kDa
GenBank蛋白编号BC047902
基因名称 LDHD
Gene ID (NCBI) 197257
RRIDAB_2249831
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86WU2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Two naturally occurring forms of lactate dehydrogenase with similar but unique substrate specificities have been isolated in lower organisms including invertebrates, fungi, and prokaryotes. These dehydrogenase enzymes are L-lactate dehydrogenase and D-lactate dehydrogenase (LDHD) that are specific to the L and D isomers of lactate, respectively (PMID: 12127981). In lactic acid bacteria, LDHD plays a key role in anaerobic energy metabolism (PMID: 497162). Despite the identification of D-lactate and other D-2-hydroxyacids in prokaryotes, and the obvious connections and similarities to vertebrate metabolic pathways, very few mammalian D-2-hydroxyacid dehydrogenases have been found. LDHD has 2 isoforms with the molecular weight of 52 and 54kDa, and can be detected as 45-54 kDa.

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