验证数据展示
经过测试的应用
| Positive WB detected in | human testis tissue, mouse brain tissue, mouse lung tissue, mouse testis tissue, A549 cells, rat testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | mouse testis tissue, human testis tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19989-1-AP targets LDHC-Specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse, pig |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lactate dehydrogenase C |
| 别名 | LDHC, LDH3, LDH X, LDH C, LDH |
| 计算分子量 | 36 kDa |
| 观测分子量 | 30-36 kDa |
| GenBank蛋白编号 | NM_002301 |
| 基因名称 | LDHC |
| Gene ID (NCBI) | 3948 |
| RRID | AB_10637847 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P07864 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LDHC, also named as CT32 and LDH-X, belongs to the LDH/MDH superfamily and LDH family. LDHC is a possible role in sperm motility. LDHC catalyzes the reaction: (S)-lactate + NAD+ = pyruvate + NADH. LDHC is a testis-specific isoform. Abnormal LDHC expression is associated with several forms of cancer. The antibody has no cross reaction with LDHA and LDHB.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LDHC-Specific antibody 19989-1-AP | Download protocol |
| IP protocol for LDHC-Specific antibody 19989-1-AP | Download protocol |
| IHC protocol for LDHC-Specific antibody 19989-1-AP | Download protocol |
| IF protocol for LDHC-Specific antibody 19989-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Pharmacol Combination of the Herbs Radix Rehmanniae and Cornus Officinalis Mitigated Testicular Damage From Diabetes Mellitus by Enhancing Glycolysis via the AGEs/RAGE/HIF-1α Axis. | ||
Andrology Generation of humanized LDHC knock-in mice as a tool to assess human LDHC-targeting contraceptive drugs | ||
Int J Biol Macromol Genome-wide analysis reveals porcine LIFR regulated by DNA methylation promotes the implantation process via the STAT3 signaling |
