Various lysates were subjected to SDS PAGE followed by western blot with 84198-3-RR (LDHA-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84198-3-PBS in a different storage buffer formulation.

WB result of LDHA-Specific antibody (84198-3-RR; 1:1000; room temperature for 1.5 hours) with wild-type and LDHA knockout MDA-MB-231 cells. This data was developed using the same antibody clone with 84198-3-PBS in a different storage buffer formulation.

The mean LDHA concentration was determined to be 61.9 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load and 6.4 ng/mL in U-251 cell extract based on a 1.2 mg/mL extract load.

Sandwich ELISA standard curve of MP03165-1, Human LDHA Recombinant Matched Antibody Pair - PBS only. 84198-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard SY00607. 84198-4-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

1x10^6 HepG2 cells were intracellularly stained with 0.25 ug LDHA-Specific Recombinant antibody (84198-3-RR, Clone:240885C7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84198-3-PBS in a different storage buffer formulation.