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验证数据展示

  • WB analysis using 84198-2-RR (same clone as 84198-2-PBS)

    WB analysis using 84198-2-RR (same clone as 84198-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84198-2-RR (LDHA-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84198-2-PBS in a different storage buffer formulation.

  • WB analysis of MDA-MB-231 using 84198-2-RR (same clone as 84198-2-PBS)

    WB analysis of MDA-MB-231 using 84198-2-RR (same clone as 84198-2-PBS)

    WB result of LDHA-Specific antibody (84198-2-RR; 1:3000; room temperature for 1.5 hours) with wild-type and LDHA knockout MDA-MB-231 cells. This data was developed using the same antibody clone with 84198-2-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 84198-2-RR (same clone as 84198-2-PBS)

    FC experiment of HepG2 using 84198-2-RR (same clone as 84198-2-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug LDHA-Specific Recombinant antibody (84198-2-RR, Clone:240885A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84198-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

LDHA-Specific Recombinant antibody, PBS Only

LDHA-Specific Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84198-2-PBS

产品说明书

CloneNo. 240885A9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), Indirect ELISA

别名

LDHA, LDH A, lactate dehydrogenase A, EC:1.1.1.27, Cell proliferation-inducing gene 19 protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84198-2-PBS targets LDHA-Specific in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lactate dehydrogenase A
别名 LDHA, LDH A, lactate dehydrogenase A, EC:1.1.1.27, Cell proliferation-inducing gene 19 protein
计算分子量 37 kDa
观测分子量35 kDa
GenBank蛋白编号NM_005566
基因名称 LDHA
Gene ID (NCBI) 3939
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP00338
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LDHA, also named as LDH-M and NY-REN-59, is an enzyme which catalyzes the inter-conversion of pyruvate and L-lactate with concomitant inter-conversion of NADH and NAD+. LDHA is found in most somatic tissues, though predominantly in muscle tissue and tumours, and belongs to the lactate dehydrogenase family. It has long been known that many human cancers have higher LDHA levels compared to normal tissues. It has also been shown that LDHA plays an important role in the development, invasion and metastasis of malignancies.