LDHA Monoclonal antibody
LDHA Monoclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, pig, rat
Applications
IF/ICC, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
2E2G6
验证数据展示
经过测试的应用
Positive WB detected in | pig liver tissue, pig skeletal muscle tissue, A431 cells, rat skeletal muscle, mouse skeletal muscle |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
66287-1-Ig targets LDHA in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.
Tested Applications | IF/ICC, IHC, WB, ELISA |
Cited Applications | WB, IP, IF, IHC |
Tested Reactivity | human, mouse, pig, rat |
Cited Reactivity | human, mouse, rat, pig |
Immunogen | LDHA fusion protein Ag21417 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | lactate dehydrogenase A |
Synonyms | lactate dehydrogenase A, LDH A, LDH M, LDH muscle subunit, LDH1, LDHA, PIG19 |
Calculated Molecular Weight | 332 aa, 37 kDa |
Observed Molecular Weight | 32-37 kDa |
GenBank Accession Number | BC067223 |
Gene Symbol | LDHA |
Gene ID (NCBI) | 3939 |
RRID | AB_2881670 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P00338 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for LDHA antibody 66287-1-Ig | Download protocol |
IHC protocol for LDHA antibody 66287-1-Ig | Download protocol |
IF protocol for LDHA antibody 66287-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Metab Augmentation of scleral glycolysis promotes myopia through histone lactylation | ||
Life Sci Aerobic exercise reduced the amount of CHRONO bound to BMAL1 and ameliorated glucose metabolic dysfunction in skeletal muscle of high-fat diet-fed mice | ||
Int J Biol Sci LAMC1-mediated preadipocytes differentiation promoted peritoneum pre-metastatic niche formation and gastric cancer metastasis. | ||
Front Oncol Lactate Dehydrogenase B Is Required for Pancreatic Cancer Cell Immortalization Through Activation of Telomerase Activity. | ||
Front Oncol Ursolic Acid Inhibits Breast Cancer Metastasis by Suppressing Glycolytic Metabolism via Activating SP1/Caveolin-1 Signaling. |