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验证数据展示

  • WB analysis using 31714-1-AP

    WB analysis using 31714-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31714-1-AP (C2orf43 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 31714-1-AP

    IHC staining of human ovary cancer using 31714-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31714-1-AP (LDAH antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LDAH Polyclonal antibody

LDAH Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31714-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

C2orf43, UPF0554 protein C2orf43, Lipid Droplet Associated Hydrolase, HLDAH

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31714-1-AP targets LDAH in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 LDAH fusion protein Ag36372 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chromosome 2 open reading frame 43
别名 C2orf43, UPF0554 protein C2orf43, Lipid Droplet Associated Hydrolase, HLDAH
观测分子量32 kDa
GenBank蛋白编号BC017473
基因名称 C2orf43
Gene ID (NCBI) 60526
RRIDAB_3670093
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9H6V9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LDAH, also known as C2orf43, is a newly identified LD protein. It has 4 isoforms and is abundantly expressed in tissues that predominantly store triacylglycerol (TAG), including liver and white and brown adipose tissue (PMID: 24357060).

实验方案

Product Specific Protocols
WB protocol for LDAH antibody 31714-1-APDownload protocol
IHC protocol for LDAH antibody 31714-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols