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LCTL Polyclonal antibody

LCTL Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 24589-1-AP

Print datasheet

Synonyms

KLG, KLPH, lactase like, Lactase like protein, LCTL

验证数据展示

  • WB analysis of MCF-7 using 24589-1-AP

    WB analysis of MCF-7 using 24589-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24589-1-AP (LCTL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 24589-1-AP

    IF Staining of HeLa using 24589-1-AP

    Immunofluorescent analysis of HeLa cells using 24589-1-AP (LCTL antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

经过测试的应用

Positive WB detected inMCF-7 cells
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24589-1-AP targets LCTL in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human
Immunogen LCTL fusion protein Ag20220 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lactase-like
Synonyms KLG, KLPH, lactase like, Lactase like protein, LCTL
Calculated Molecular Weight 567 aa, 65 kDa
Observed Molecular Weight 39 kDa, 65 kDa, 70 kDa
GenBank Accession NumberBC152990
Gene Symbol LCTL
Gene ID (NCBI) 197021
RRIDAB_2879625
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UWM7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lactase-like is a glycosidase enzyme, which could hydrolyze glycosidic bonds and are classified into families based on primary amino acid sequence. The mouse ortholog of this protein has been characterized and has a domain structure of an N-terminal signal peptide, glycosidase domain, transmembrane domain, and a short cytoplasmic tail.

实验方案

Product Specific Protocols
WB protocol for LCTL antibody 24589-1-APDownload protocol
IF protocol for LCTL antibody 24589-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols