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验证数据展示

  • WB analysis of MCF-7 using 24589-1-AP

    WB analysis of MCF-7 using 24589-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24589-1-AP (LCTL Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 24589-1-AP

    IF Staining of HeLa using 24589-1-AP

    Immunofluorescent analysis of HeLa cells using 24589-1-AP (LCTL antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

LCTL Polyclonal antibody

LCTL Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 24589-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

KLG, KLPH, lactase like, Lactase like protein, LCTL

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24589-1-AP targets LCTL in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag20220

Product name: Recombinant human LCTL protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 250-541 aa of BC152990

Sequence: SKQQGLVGISLNCDWGEPVDISNPKDLEAAERYLQFCLGWFANPIYAGDYPQVMKDYIGRKSAEQGLEMSRLPVFSLQEKSYIKGTSDFLGLGHFTTRYITERNYPSRQGPSYQNDRDLIELVDPNWPDLGSKWLYSVPWGFRRLLNFAQTQYGDPPIYVMENGASQKFHCTQLCDEWRIQYLKGYINEMLKAIKDGANIKGYTSWSLLDKFEWEKGYSDRYGFYYVEFNDRNKPRYPKASVQYYKKIIIANGFPNPREVESWYLKALETCSINNQMLAAEPLLSHMQMVTE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lactase-like
别名 KLG, KLPH, lactase like, Lactase like protein, LCTL
计算分子量 567 aa, 65 kDa
观测分子量 39 kDa, 65 kDa, 70 kDa
GenBank蛋白编号BC152990
基因名称 LCTL
Gene ID (NCBI) 197021
RRIDAB_2879625
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UWM7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lactase-like is a glycosidase enzyme, which could hydrolyze glycosidic bonds and are classified into families based on primary amino acid sequence. The mouse ortholog of this protein has been characterized and has a domain structure of an N-terminal signal peptide, glycosidase domain, transmembrane domain, and a short cytoplasmic tail.

实验方案

Product Specific Protocols
WB protocol for LCTL antibody 24589-1-APDownload protocol
IF protocol for LCTL antibody 24589-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols