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LCE1B Polyclonal antibody

LCE1B Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 21771-1-AP

Print datasheet

Synonyms

late cornified envelope 1B, Late envelope protein 2, LCE1B, LEP2, SPRL2A

验证数据展示

  • WB analysis of A375 using 21771-1-AP

    WB analysis of A375 using 21771-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 21771-1-AP (LCE1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin using 21771-1-AP

    IHC staining of human skin using 21771-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 21771-1-AP (LCE1B antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skin using 21771-1-AP

    IHC staining of human skin using 21771-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 21771-1-AP (LCE1B antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of A375 using 21771-1-AP

    IF Staining of A375 using 21771-1-AP

    Immunofluorescent analysis of A375 cells using 21771-1-AP (LCE1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA375 cells, mouse skin tissue, rat skin tissue
Positive IHC detected inhuman skin tissue, human oesophagus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21771-1-AP is LCE1B Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen LCE1B fusion protein Ag16442 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name late cornified envelope 1B
Synonyms late cornified envelope 1B, Late envelope protein 2, LCE1B, LEP2, SPRL2A
Calculated Molecular Weight 118 aa, 12 kDa
Observed Molecular Weight 10-12 kDa
GenBank Accession NumberBC104232
Gene Symbol LCE1B
Gene ID (NCBI) 353132
RRIDAB_10858482
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5T7P3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human LCE1B (Late cornified envelope protein 1B), also named late envelope protein 2 (LEP2), is located at human Chromosome1q21 which contains multiple LCE clusters. LCE1B gene is predicated to encode a 118-aa protein, and is currently evidenced at transcript level. Its expression was skin-specific and was readily detected in adult trunk skin, adult arm skin, fetal skin, penal skin, vulva, esophagus and tongue (PMID: 15854049). LCE genes respond to environmental stimuli such as calcium levels and ultraviolet (UV) light, however, LCE groups have distinct functions (PMID: 15854049).

实验方案

Product Specific Protocols
WB protocol for LCE1B antibody 21771-1-APDownload protocol
IHC protocol for LCE1B antibody 21771-1-APDownload protocol
IF protocol for LCE1B antibody 21771-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols