验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
12243-1-AP targets LCAT in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag2887 Product name: Recombinant human LCAT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 94-440 aa of BC014781 Sequence: LGVDCWIDNTRVVYNRSSGLVSNAPGVQIRVPGFGKTYSVEYLDSSKLAGYLHTLVQNLVNNGYVRDETVRAAPYDWRLEPGQQEEYYRKLAGLVEEMHAAYGKPVFLIGHSLGCLHLLYFLLRQPQAWKDRFIDGFISLGAPWGGSIKPMLVLASGDNQGIPIMSSIKLKEEQRITTTSPWMFPSRMAWPEDHVFISTPSFNYTGRDFQRFFADLHFEEGWYMWLQSRDLLAGLPAPGVEVYCLYGVGLPTPRTYIYDHGFPYTDPVGVLYEDGDDTVATRSTELCGLWQGRQPQPVHLLPLHGIQHLNMVFSNLTLEHINAILLGAYRQGPPASPTASPEPPPPE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | lecithin-cholesterol acyltransferase |
| 别名 | 1-alkyl-2-acetylglycerophosphocholine esterase, EC:2.3.1.43, EC:3.1.1.47, Lecithin-cholesterol acyltransferase, Phosphatidylcholine-sterol acyltransferase |
| 计算分子量 | 440 aa, 50 kDa |
| 观测分子量 | 55 kDa |
| GenBank蛋白编号 | BC014781 |
| 基因名称 | LCAT |
| Gene ID (NCBI) | 3931 |
| RRID | AB_10638476 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P04180 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LCAT (Lecithin-cholesterol acyltransferase) is a lipoprotein-associated enzyme responsible for the synthesis of most of the plasma cholesteryl esters (CE), and as a consequence, for the maturation of high density-lipoproteins (HDL). LCAT is a 416 amino acid protein that binds to lipoproteins or is present in lipid-free form in plasma and is secreted by the liver in humans (PMID: 34256778, 26607351). Both mice and rabbits transgenic for human LCAT have marked increases in plasma HDL cholesterol and apolipoprotein (apo) A-I levels (PMID: 9054454).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for LCAT antibody 12243-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Physiol Biochem Ganoderma Lucidum Polysaccharide Peptide Alleviates Hepatoteatosis via Modulating Bile Acid Metabolism Dependent on FXR-SHP/FGF. | ||
Invest Ophthalmol Vis Sci Alteration in Lysophospholipids and Converting Enzymes in Glaucomatous Optic Nerves. | ||
Lipids Health Dis Chronic unpredictable mild stress combined with a high-fat diets aggravates atherosclerosis in rats. | ||
Heliyon The anti-hyperlipidemia effect of Atractylodes macrocephala Rhizome increased HDL via reverse cholesterol transfer |
