LC3A/B Monoclonal antibody

LC3A/B Monoclonal Antibody for ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, mouse and More (2)

Applications

ELISA and More (4)

Conjugate

Unconjugated

CloneNo.

2B2B5

Cat No : 66139-1-Ig

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Synonyms

LC3, LC3A, LC3A/B, LC3B, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, MAP1ALC3, MAP1BLC3, MAP1LC3A, MAP1LC3A/B, MAP1LC3B



经过测试的应用

推荐稀释比

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 66139-1-Ig is LC3A/B Fusion Protein expressed in E. coli.

Tested Applications ELISA
Cited Applications IF, IHC, IP, WB
Tested Reactivity human, pig, mouse
Cited Reactivityhuman, mouse, rat, hamster, pig
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name microtubule-associated protein 1 light chain 3 alpha
Synonyms LC3, LC3A, LC3A/B, LC3B, MAP1A/MAP1B LC3 A, MAP1A/MAP1B light chain 3 A, MAP1ALC3, MAP1BLC3, MAP1LC3A, MAP1LC3A/B, MAP1LC3B
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14 kDa, 16 kDa
GenBank Accession NumberNM_032514
Gene Symbol MAP1LC3A
Gene ID (NCBI) 84557
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9H492
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LC3, also known as microtubule-associated protein 1 light chain 3 (MAP1LC3), is a mammalian homolog of the yeast Atg8p (autophagy-related 8 protein) that is involved in autophagy process. Three isoforms of LC3 exist as LC3A,LC3B, and LC3C. LC3 is first synthesized as pro-LC3, and undergoes C-terminal cleavage to yield a cytosolic precursor known as LC3-I. During autophagy LC3-I is subsequently conjugated to PE lipid as lipidated form: LC3-II. The lipidated form migrates faster on SDS-PAGE gel. The increased the level of LC3-II has been widely used as autophagy marker. This antibody recognizes both LC3-I and LC3-II.

发表文章

SpeciesApplicationTitle
mouseIF

Cell Res

NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.

Authors - Min Liu
mouse,ratWB,IP

Cell Death Dis

Exogenous H2S facilitating ubiquitin aggregates clearance via autophagy attenuates type 2 diabetes-induced cardiomyopathy.

Authors - Jichao Wu
pigIF

Cell Prolif

S-nitrosoglutathione reductase maintains mitochondrial homeostasis by promoting clearance of damaged mitochondria in porcine preimplantation embryos.

Authors - Ying-Jie Niu
mouseIF

Mol Neurobiol

Colistin-Induced Apoptosis of Neuroblastoma-2a Cells Involves the Generation of Reactive Oxygen Species, Mitochondrial Dysfunction, and Autophagy.

Authors - Chongshan Dai
pigWB

Aging (Albany NY)

Ubiquinol-10 delays postovulatory oocyte aging by improving mitochondrial renewal in pigs.

Authors - Ying-Jie Niu
hamsterIF

Front Mol Neurosci

Downregulation of the Repressor Element 1-Silencing Transcription Factor (REST) Is Associated with Akt-mTOR and Wnt-β-Catenin Signaling in Prion Diseases Models.

Authors - Zhiqi Song