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验证数据展示

  • WB analysis using 85900-1-RR

    WB analysis using 85900-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85900-1-RR (LAYN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 85900-1-RR

    IHC staining of mouse brain using 85900-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85900-1-RR (LAYN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 85900-1-RR

    IF Staining of A549 using 85900-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using LAYN antibody (85900-1-RR, Clone: 250175B7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

LAYN Recombinant antibody

LAYN Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 85900-1-RR

产品说明书

CloneNo. 250175B7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Layilin, UNQ208/PRO234

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRaji cells, HeLa cells, A549 cells, NCI-H1299 cells, A172 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85900-1-RR targets LAYN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 LAYN fusion protein Ag14508 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 layilin
别名 Layilin, UNQ208/PRO234
计算分子量 382 aa, 43 kDa
观测分子量43 kDa
GenBank蛋白编号BC025407
基因名称 LAYN
Gene ID (NCBI) 143903
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ6UX15
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LAYN acts as a receptor for hyaluronic acid (HA) and is involved in various cellular processes, including cell adhesion, movement, and signal transduction. In the context of cancer, LAYN has been identified as a prognostic biomarker. High expression of LAYN is associated with poor prognosis and increased immune cell infiltration in various cancers, such as colorectal cancer and gastric cancer. It may also play a role in T-cell exhaustion and the regulation of tumor-associated macrophages (PMID: 30761122).

实验方案

Product Specific Protocols
WB protocol for LAYN antibody 85900-1-RRDownload protocol
IHC protocol for LAYN antibody 85900-1-RRDownload protocol
IF protocol for LAYN antibody 85900-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols