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验证数据展示

  • WB analysis using 21557-1-AP

    WB analysis using 21557-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21557-1-AP (LAX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 21557-1-AP

    IHC staining of human spleen using 21557-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 21557-1-AP (LAX1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human spleen using 21557-1-AP

    IHC staining of human spleen using 21557-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 21557-1-AP (LAX1 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 21557-1-AP

    IF Staining of HeLa using 21557-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 21557-1-AP (LAX1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

LAX1 Polyclonal antibody

LAX1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21557-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

LAX, LAX1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

21557-1-AP targets LAX1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 LAX1 fusion protein Ag15599 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lymphocyte transmembrane adaptor 1
别名 LAX, LAX1
计算分子量 398 aa, 44 kDa
观测分子量 44 kDa
GenBank蛋白编号BC069650
基因名称 LAX1
Gene ID (NCBI) 54900
RRIDAB_10732821
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IWV1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LAX1 antibody 21557-1-APDownload protocol
IHC protocol for LAX1 antibody 21557-1-APDownload protocol
IF protocol for LAX1 antibody 21557-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Immunol

Mettl14-Mediated m6A Modification Is Essential for Germinal Center B Cell Response.

Authors - Hengjun Huang
View Article
humanWB

Cell Rep Med

A target discovery pipeline identified ILT3 as a target for immunotherapy of multiple myeloma

Authors - Francesco Di Meo
View Article