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验证数据展示

  • IF Staining of A549 using 68080-1-Ig (same clone as 68080-1-PBS)

    IF Staining of A549 using 68080-1-Ig (same clone as 68080-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using LASP1 antibody (68080-1-Ig, Clone: 1G4B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

  • WB analysis using 68080-1-Ig (same clone as 68080-1-PBS)

    WB analysis using 68080-1-Ig (same clone as 68080-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68080-1-Ig (LASP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 68080-1-Ig (same clone as 68080-1-PBS)

    IHC staining of human liver cancer using 68080-1-Ig (same clone as 68080-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68080-1-Ig (LASP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LASP1 antibody included
  • IHC staining of human liver cancer using 68080-1-Ig (same clone as 68080-1-PBS)

    IHC staining of human liver cancer using 68080-1-Ig (same clone as 68080-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68080-1-Ig (LASP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LASP1 antibody included
  • IHC staining of human breast cancer using 68080-1-Ig (same clone as 68080-1-PBS)

    IHC staining of human breast cancer using 68080-1-Ig (same clone as 68080-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68080-1-Ig (LASP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LASP1 antibody included
  • IHC staining of human breast cancer using 68080-1-Ig (same clone as 68080-1-PBS)

    IHC staining of human breast cancer using 68080-1-Ig (same clone as 68080-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68080-1-Ig (LASP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68080-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with LASP1 antibody included
  • Featured Product
  • KD/KO验证

LASP1 Monoclonal antibody, PBS Only

LASP1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68080-1-PBS

产品说明书

CloneNo. 1G4B6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

1G4B6, LASP-1, LIM and SH3 protein 1, MLN50

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy LASP1 Ready-To-Use IHC Kit. LASP1 primary antibody included.

产品信息

68080-1-PBS targets LASP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag18101

Product name: Recombinant human LASP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 125-210 aa of BC012460

Sequence: EFEKSRMGPSGGEGMEPERRDSQDGSSYRRPLEQQQPHHIPTSAPVYQQPQQQPVAQSYGGYKEPAAPVSIQRSAPGGGGKRYRAA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 LIM and SH3 protein 1
别名 1G4B6, LASP-1, LIM and SH3 protein 1, MLN50
计算分子量 30 kDa
观测分子量35-38 kDa
GenBank蛋白编号BC012460
基因名称 LASP1
Gene ID (NCBI) 3927
ENSEMBL Gene IDENSG00000002834
RRIDAB_2918817
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ14847
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LASP1(LIM and SH3 protein 1), also known as MLN50, is a 261 amino acid protein that localizes to both the cytoplasm and the cytoskeleton(PMID: 7589475). LASP1 consists of an N-terminal LIM-domain with two zinc finger motifs, followed by two central actin-binding nebulin repeats, flanked by a linker region and a C-terminal SH3 domain (PMID: 17177073, 9848085). LASP-1 interacts with F-Actin and plays an important role in the regulation of Actin-associated cytoskeletal organization. Agonist-dependent changes in LASP1 phosphorylation may regulate Actin-related ion transport activities in epithelial cells (PMID: 15465019,12571245). Overexpression of LASP-1 is associated with breast cancer, and plays a role in tumor transformation and metastasis (PMID: 17956604).

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