验证数据展示

WB analysis of PC-3 using 10515-1-AP
WB result of LASP1 antibody (10515-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LASP1 transfected PC-3 cells.
IP experiment of A549 using 10515-1-AP
IP result of anti-LASP1 (IP:10515-1-AP, 4ug; Detection:10515-1-AP 1:8000) with A549 cells lysate 600 ug.
WB analysis using 10515-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10515-1-AP (LASP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 10515-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10515-1-AP (LASP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with LASP1 antibody included
IHC staining of human colon cancer using 10515-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10515-1-AP (LASP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with LASP1 antibody included
IHC staining of human liver cancer using 10515-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10515-1-AP (LASP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with LASP1 antibody included
IHC staining of human liver cancer using 10515-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10515-1-AP (LASP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with LASP1 antibody included
IF Staining of A549 using 10515-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using LASP1 antibody (10515-1-AP) at dilution of 1:1600 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO验证

LASP1 Polyclonal antibody

LASP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10515-1-AP

产品说明书

引用文献(33)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

别名

MLN50, LIM and SH3 protein 1, LASP-1

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

规格	价格	库存

点击咨询售前客服

点击咨询售后客服

概述

经过测试的应用
产品信息
发表文章 (33)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	A431 cells, MDA-MB-453s cells, mouse brain tissue, PC-3 cells, MCF-7 cells, Jurkat cells, SGC-7901 cells, SKOV-3 cells
Positive IP detected in	A549 cells
Positive IHC detected in	human colon cancer tissue, human breast cancer tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A549 cells

Planning an IHC experiment? We recommend our IHCeasy LASP1 Ready-To-Use IHC Kit. LASP1 primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:800-1:3200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 10 publications below
WB	See 29 publications below
IHC	See 13 publications below
IF	See 7 publications below
IP	See 4 publications below
CoIP	See 1 publications below

产品信息

10515-1-AP targets LASP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用	WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, CoIP
经测试反应性	human, mouse
文献引用反应性	human, mouse, rat
免疫原	CatNo: Ag0800 Product name: Recombinant human LASP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 125-210 aa of BC012460 Sequence: EFEKSRMGPSGGEGMEPERRDSQDGSSYRRPLEQQQPHHIPTSAPVYQQPQQQPVAQSYGGYKEPAAPVSIQRSAPGGGGKRYRAA 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	LIM and SH3 protein 1
别名	MLN50, LIM and SH3 protein 1, LASP-1
计算分子量	30 kDa
观测分子量	38-40 kDa
GenBank蛋白编号	BC012460
基因名称	LASP1
Gene ID (NCBI)	3927
ENSEMBL Gene ID	ENSG00000002834
RRID	AB_2280966
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q14847
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

LASP1(LIM and SH3 protein 1), also known as MLN50, is a 261 amino acid protein that localizes to both the cytoplasm and the cytoskeleton(PMID: 7589475). LASP1 consists of an N-terminal LIM-domain with two zinc finger motifs, followed by two central actin-binding nebulin repeats, flanked by a linker region and a C-terminal SH3 domain (PMID: 17177073, 9848085). LASP-1 interacts with F-Actin and plays an important role in the regulation of Actin-associated cytoskeletal organization. Agonist-dependent changes in LASP1 phosphorylation may regulate Actin-related ion transport activities in epithelial cells (PMID: 15465019,12571245). Overexpression of LASP-1 is associated with breast cancer, and plays a role in tumor transformation and metastasis (PMID: 17956604).

实验方案

Product Specific Protocols
IF protocol for LASP1 antibody 10515-1-AP	Download protocol
IHC protocol for LASP1 antibody 10515-1-AP	Download protocol
IP protocol for LASP1 antibody 10515-1-AP	Download protocol
WB protocol for LASP1 antibody 10515-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
	IP	Cell Host Microbe Global mapping of Salmonella enterica-host protein-protein interactions during infection. Authors - Philipp Walch View Article
human	WB	J Exp Clin Cancer Res RNA-binding protein CCDC137 activates AKT signaling and promotes hepatocellular carcinoma through a novel non-canonical role of DGCR8 in mRNA localization Authors - Shuang Tao View Article
human	WB,IHC	Oncogene LASP1 interacts with N-WASP to activate the Arp2/3 complex and facilitate colorectal cancer metastasis by increasing tumour budding and worsening the pattern of invasion. Authors - Pingping Yan View Article
human	WB	Oncotarget Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma. Authors - Hao Bo View Article
human	WB,IHC,IF,CoIP	Oncogenesis CCT8 recovers WTp53-suppressed cell cycle evolution and EMT to promote colorectal cancer progression. Authors - Qing Liao KD Validated View Article
human	WB,IHC,IF	Cancer Lett LASP-1 induces proliferation, metastasis and cell cycle arrest at the G2/M phase in gallbladder cancer by down-regulating S100P via the PI3K/AKT pathway. Authors - ZhiZhen Li KD Validated View Article

验证数据展示

WB analysis of PC-3 using 10515-1-AP

IP experiment of A549 using 10515-1-AP

WB analysis using 10515-1-AP

IHC staining of human colon cancer using 10515-1-AP

IHC staining of human colon cancer using 10515-1-AP

IHC staining of human liver cancer using 10515-1-AP

IHC staining of human liver cancer using 10515-1-AP

IF Staining of A549 using 10515-1-AP

LASP1 Polyclonal antibody

LASP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Global mapping of Salmonella enterica-host protein-protein interactions during infection.

RNA-binding protein CCDC137 activates AKT signaling and promotes hepatocellular carcinoma through a novel non-canonical role of DGCR8 in mRNA localization

LASP1 interacts with N-WASP to activate the Arp2/3 complex and facilitate colorectal cancer metastasis by increasing tumour budding and worsening the pattern of invasion.

Upregulated long non-coding RNA AFAP1-AS1 expression is associated with progression and poor prognosis of nasopharyngeal carcinoma.

CCT8 recovers WTp53-suppressed cell cycle evolution and EMT to promote colorectal cancer progression.

LASP-1 induces proliferation, metastasis and cell cycle arrest at the G2/M phase in gallbladder cancer by down-regulating S100P via the PI3K/AKT pathway.