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LAS1L Polyclonal antibody

LAS1L Polyclonal Antibody for IF/ICC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 16010-1-AP

Print datasheet

Synonyms

LAS1 like (S. cerevisiae), LAS1L, Protein LAS1 homolog

验证数据展示

  • WB analysis using 16010-1-AP

    WB analysis using 16010-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16010-1-AP (LAS1L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 16010-1-AP

    IP experiment of HEK-293 using 16010-1-AP

    IP result of anti-LAS1L (IP:16010-1-AP, 4ug; Detection:16010-1-AP 1:1000) with HEK-293 cells lysate 1200ug.

  • IF Staining of HepG2 using 16010-1-AP

    IF Staining of HepG2 using 16010-1-AP

    Immunofluorescent analysis of HepG2 cells using 16010-1-AP (LAS1L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inCOLO 320 cells, HEK-293 cells, HeLa cells, HepG2 cells, PC-3 cells
Positive IP detected inHEK-293 cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

16010-1-AP targets LAS1L in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen LAS1L fusion protein Ag8732 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name LAS1-like (S. cerevisiae)
Synonyms LAS1 like (S. cerevisiae), LAS1L, Protein LAS1 homolog
Calculated Molecular Weight 734 aa, 83 kDa
Observed Molecular Weight 83 kDa, 70-75 kDa
GenBank Accession NumberBC014545
Gene Symbol LAS1L
Gene ID (NCBI) 81887
RRIDAB_2132810
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4W2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LAS1L is a nucleolar protein required for the synthesis of the 60S ribosomal subunit and maturation of the 28S rRNA. LAS1L interacts with PELP1, TEX10, and WDR18, along with NOL9 and SENP3, to form a novel nucleolar complex that cofractionates with the 60S preribosomal subunit.

实验方案

Product Specific Protocols
WB protocol for LAS1L antibody 16010-1-APDownload protocol
IF protocol for LAS1L antibody 16010-1-APDownload protocol
IP protocol for LAS1L antibody 16010-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation.

Authors - Fanis Pavlos P
View Article
humanWB

Breast Cancer Res Treat

Targeting PELP1 oncogenic signaling in TNBC with the small molecule inhibitor SMIP34

Authors - Kristin A Altwegg
View Article
humanWB

Mol Oncol

PELP1 inhibition by SMIP34 reduces endometrial cancer progression via attenuation of ribosomal biogenesis

Authors - Xue Yang
View Article