验证数据展示
经过测试的应用
Positive WB detected in | LNCaP cells, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
84950-4-RR targets LARP7 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | LARP7 fusion protein Ag10850 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | La ribonucleoprotein domain family, member 7 |
别名 | PIP7S, La-related protein 7, La related protein 7, hLARP7, HDCMA18P |
计算分子量 | 582 aa, 67 kDa |
观测分子量 | 67 kDa |
GenBank蛋白编号 | BC006981 |
基因名称 | LARP7 |
Gene ID (NCBI) | 51574 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | Q4G0J3 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LARP7 belongs to the LARP RNA-binding protein family, which includes the lupus LA antigen gene, and modulates the metabolism and function of a variety of RNA species [PMID:20138158]. LARP7 binds to the 7SK RNP complex, and sequesters the positive transcription elongation factor b (P-TEFb) in an inactive form, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation.[PMID:19416841].
实验方案
Product Specific Protocols | |
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WB protocol for LARP7 antibody 84950-4-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |