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LARP7 Polyclonal antibody

LARP7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17067-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (2)

P-TEFb-interaction protein for 7SK stability, PIP7S, La-related protein 7, La related protein 7, hLARP7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, human brain tissue, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human placenta tissue, human testis tissue, human skin tissue, human liver tissue, human spleen tissue, human stomach cancer tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy LARP7 Ready-To-Use IHC Kit. LARP7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17067-1-AP targets LARP7 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 LARP7 fusion protein Ag10891 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 La ribonucleoprotein domain family, member 7
别名 P-TEFb-interaction protein for 7SK stability, PIP7S, La-related protein 7, La related protein 7, hLARP7
计算分子量 582 aa, 67 kDa
观测分子量 67 kDa
GenBank蛋白编号BC006981
基因名称 LARP7
Gene ID (NCBI) 51574
RRIDAB_2132693
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ4G0J3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LARP7 belongs to the LARP RNA-binding protein family, which includes the lupus LA antigen gene, and modulates the metabolism and function of a variety of RNA species [PMID:20138158]. LARP7 binds to the 7SK RNP complex, and sequesters the positive transcription elongation factor b (P-TEFb) in an inactive form, preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. binds to and stabilizes 7sk snRNA[PMID:19416841].

实验方案

Product Specific Protocols
WB protocol for LARP7 antibody 17067-1-APDownload protocol
IHC protocol for LARP7 antibody 17067-1-APDownload protocol
IF protocol for LARP7 antibody 17067-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Genes Dev

Cell cycle gene-specific control of transcription has a critical role in proliferation of primordial germ cells.

Authors - Daiji Okamura
mouse,humanIF,IP

Nat Commun

Interaction of 7SK with the Smn complex modulates snRNP production.

Authors - Changhe Ji
  • KD Validated
humanWB

Nucleic Acids Res

7SK small nuclear RNA directly affects HMGA1 function in transcription regulation.

Authors - Eilebrecht Sebastian S
humanWB

Nucleic Acids Res

HMGA1 recruits CTIP2-repressed P-TEFb to the HIV-1 and cellular target promoters.

Authors - Sebastian Eilebrecht
humanWB

EMBO Rep

Human METTL16 is a N6-methyladenosine (m6A) methyltransferase that targets pre-mRNAs and various non-coding RNAs.

Authors - Ahmed S Warda
humanWB,IF,IP

MBio

Interaction of the Ankyrin H Core Effector of Legionella with the Host LARP7 Component of the 7SK snRNP Complex.

Authors - Juanita Von Dwingelo
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