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验证数据展示

  • WB analysis using 30627-1-AP

    WB analysis using 30627-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30627-1-AP (LAPTM4A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse kidney using 30627-1-AP

    IF Staining of mouse kidney using 30627-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using LAPTM4A antibody (30627-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse kidney using 30627-1-AP

    IF Staining of mouse kidney using 30627-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using LAPTM4A antibody (30627-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

LAPTM4A Polyclonal antibody

LAPTM4A Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 30627-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF-P, ELISA

别名

Golgi 4-transmembrane-spanning transporter MTP, HUMORF13, KIAA0108, LAPTM4, Lysosomal-associated transmembrane protein 4A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse liver tissue
Positive IF-P detected inmouse kidney tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30627-1-AP targets LAPTM4A in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag6501

Product name: Recombinant human LAPTM4A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-233 aa of BC000421

Sequence: MVSMSFKRNRSDRFYSTRCCGCCHVRTGTIILGTWYMVVNLLMAILLTVEVTHPNSMPAVNIQYEVIGNYYSSERMADNACVLFAVSVLMFIISSMLVYGAISYQVGWLIPFFCYRLFDFVLSCLVAISSLTYLPRIKEYLDQLPDFPYKDDLLALDSSCLLFIVLVFFALFIIFKAYLINCVWNCYKYINNRNVPEIAVYPAFEAPPQYVLPTYEMAVKMPEKEPPPPYLPA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lysosomal protein transmembrane 4 alpha
别名 Golgi 4-transmembrane-spanning transporter MTP, HUMORF13, KIAA0108, LAPTM4, Lysosomal-associated transmembrane protein 4A
计算分子量 27 kDa
观测分子量70 kDa
GenBank蛋白编号BC000421
基因名称 LAPTM4A
Gene ID (NCBI) 9741
RRIDAB_3086376
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15012
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LAPTM4A is an intracellular membrane protein that regulates the function of organic cation transporter-2 (OCT2) through direct interaction. LAPTM4A can also form a complex with A4GALT in the Golgi and shows at about 70 kDa.

实验方案

Product Specific Protocols
IF protocol for LAPTM4A antibody 30627-1-APDownload protocol
WB protocol for LAPTM4A antibody 30627-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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