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验证数据展示

  • IF Staining of HeLa using 66417-1-Ig (same clone as 66417-1-PBS)

    IF Staining of HeLa using 66417-1-Ig (same clone as 66417-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using LAP3 antibody (66417-1-Ig, Clone: 1B11B8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66417-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 66417-1-Ig (same clone as 66417-1-PBS)

    WB analysis of NIH/3T3 using 66417-1-Ig (same clone as 66417-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66417-1-Ig (LAP3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66417-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung using 66417-1-Ig (same clone as 66417-1-PBS)

    IHC staining of human lung using 66417-1-Ig (same clone as 66417-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66417-1-Ig (LAP3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66417-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung using 66417-1-Ig (same clone as 66417-1-PBS)

    IHC staining of human lung using 66417-1-Ig (same clone as 66417-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66417-1-Ig (LAP3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66417-1-PBS in a different storage buffer formulation.

LAP3 Monoclonal antibody, PBS Only

LAP3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66417-1-PBS

产品说明书

CloneNo. 1B11B8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Cytosol aminopeptidase, LAP, LAP 3, LAP3, LAPEP, leucine aminopeptidase 3, Leucyl aminopeptidase, PEPS, Peptidase S, Proline aminopeptidase, Prolyl aminopeptidase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66417-1-PBS targets LAP3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag6300

Product name: Recombinant human LAP3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 271-519 aa of BC065564

Sequence: NANEPPLVFVGKGITFDSGGISIKASANMDLMRADMGGAATICSAIVSAAKLNLPINIIGLAPLCENMPSGKANKPGDVVRAKNGKTIQVDNTDAEGRLILADALCYAHTFNPKVILNAATLTGAMDVALGSGATGVFTNSSWLWNKLFEASIETGDRVWRMPLFEHYTRQVVDCQLADVNNIGKYRSAGACTAAAFLKEFVTHPKWAHLDIAGVMTNKDEVPYLRKGMTGRPTRTLIEFLLRFSQDNA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 leucine aminopeptidase 3
别名 Cytosol aminopeptidase, LAP, LAP 3, LAP3, LAPEP, leucine aminopeptidase 3, Leucyl aminopeptidase, PEPS, Peptidase S, Proline aminopeptidase, Prolyl aminopeptidase
计算分子量 56 kDa
观测分子量 56 kDa
GenBank蛋白编号BC065564
基因名称 LAP3
Gene ID (NCBI) 51056
RRIDAB_2881789
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP28838
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LAP3(Leucine aminopeptidase 3) is also named as LAPEP, PEPS(peptidase S) and belongs to the peptidase M17 family. It can catalyzes the removal of unsubstituted N-terminal amino-acids from various peptides and be presumably involved in the processing end regular turnover of intracellular proteins. PEPS is found in a wide variety of tissues and cultured cells but not in red cells and skin. The enzyme can utilize several di-, tri-, and tetrapeptides as substrates and is the slowest migrating of the peptidases after electrophoresis.(PMID:689684). LAP3 has two isoform with MW of 52 and 56 kDa.

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