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验证数据展示

  • WB analysis using 68160-1-Ig

    WB analysis using 68160-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68160-1-Ig (LANCL1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of HeLa using 68160-1-Ig

    WB analysis of HeLa using 68160-1-Ig

    WB result of LANCL1 antibody (68160-1-Ig; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LANCL1 transfected HeLa cells.

  • IP experiment of mouse brain using 68160-1-Ig

    IP experiment of mouse brain using 68160-1-Ig

    IP result of anti-LANCL1 (IP:68160-1-Ig, 5ug; Detection:68160-1-Ig 1:2000) with mouse brain tissue lysate 1600 ug.

  • IF Staining of U-251 using 68160-1-Ig

    IF Staining of U-251 using 68160-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using LANCL1 antibody (68160-1-Ig, Clone: 2H6E8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO验证

LANCL1 Monoclonal antibody

LANCL1 Monoclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 68160-1-Ig

产品说明书

CloneNo. 2H6E8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IF/ICC, IP, ELISA

别名

2H6E8, EC:2.5.1.18, Glutathione S-transferase LANCL1, GPR69A, LanC like protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IF/ICC detected inU-251 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68160-1-Ig targets LANCL1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag32665

Product name: Recombinant human LANCL1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 100-399 aa of BC028685

Sequence: TKRSITFLCGDAGPLAVAAVLYHKMNNEKQAEDCITRLIHLNKIDPHAPNEMLYGRIGYIYALLFVNKNFGVEKIPQSHIQQICETILTSGENLARKRNFTAKSPLMYEWYQEYYVGAAHGLAGIYYYLMQPSLQVSQGKLHSLVKPSVDYVCQLKFPSGNYPPCIGDNRDLLVHWCHGAPGVIYMLIQAYKVFREEKYLCDAYQCADVIWQYGLLKKGYGLCHGSAGNAYAFLTLYNLTQDMKYLYRACKFAEWCLEYGEHGCRTPDTPFSLFEGMAGTIYFLADLLVPTKARFPAFEL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 LanC lantibiotic synthetase component C-like 1 (bacterial)
别名 2H6E8, EC:2.5.1.18, Glutathione S-transferase LANCL1, GPR69A, LanC like protein 1
计算分子量 399 aa, 45 kDa
观测分子量40 kDa
GenBank蛋白编号BC028685
基因名称 LANCL1
Gene ID (NCBI) 10314
RRIDAB_2923679
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO43813
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LanCL1 ( also known as P40 or GRP69A), is homologous to bacterial lanthionine synthetase C (LanC) family, which is involved in the biosynthesis of antimicrobial peptides (lantibiotics). LanCL1 was identified as a reduced glutathione (GSH)-binding protein and primarily expressed in neural tissues and testis.

实验方案

Product Specific Protocols
WB protocol for LANCL1 antibody 68160-1-IgDownload protocol
IF protocol for LANCL1 antibody 68160-1-IgDownload protocol
IP protocol for LANCL1 antibody 68160-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols