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验证数据展示

  • WB analysis of human testis using 67261-1-Ig

    WB analysis of human testis using 67261-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 67261-1-Ig (LAMC3-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse testis using 67261-1-Ig

    IF Staining of mouse testis using 67261-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using LAMC3-Specific antibody (67261-1-Ig, Clone: 4B8C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Affinity and Kinetic Characterization of 67261-1-Ig

    Affinity and Kinetic Characterization of 67261-1-Ig

    Biolayer interferometry (BLl) kinetic assays of 67261-1-PBS against Human GRP78/BIP were performed. The affinity constant is 0.559 nM.

LAMC3-Specific Monoclonal antibody

LAMC3-Specific Monoclonal Antibody for WB, IF-P, ELISA
Cat No. 67261-1-Ig

产品说明书

CloneNo. 4B8C5
引用文献(1)

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

WB, IF-P, ELISA

别名

LAMC3, 4B8C5, Laminin 12 subunit gamma, Laminin 14 subunit gamma, Laminin 15 subunit gamma

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman testis tissue, mouse placenta tissue, PC-3 cells
Positive IF-P detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67261-1-Ig targets LAMC3-Specific in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF-P, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag19568

Product name: Recombinant human LAMC3-Specific protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1225-1575 aa of NM_006059

Sequence: VAEVLPEAESVLATVQQVGADTAPYLALLASPGALPQKSRAEDLGLKAKALEKTVASWQHMATEAARTLQTAAQATLRQTEPLTKLHQEARAALTQASSSVQAATVTVMGARTLLADLEGMKLQFPRPKDQAALQRKADSVSDRLLADTRKKTKQAERMLGNAAPLSSSAKKKGREAEVLAKDSAKLAKALLRERKQAHRRASRLTSQTQATLQQASQQVLASEARRQELEEAERVGAGLSEMEQQIRESRISLEKDIETLSELLARLGSLDTHQAPAQALNETQWALERLRLQLGSPGSLQRKLSLLEQESQQQELQIQGFESDLAEIRADKQNLEAILHSLPENCASWQ

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 laminin, gamma 3
别名 LAMC3, 4B8C5, Laminin 12 subunit gamma, Laminin 14 subunit gamma, Laminin 15 subunit gamma
计算分子量 1575 aa, 171 kDa
观测分子量 180-200 kDa
GenBank蛋白编号NM_006059
基因名称 LAMC3
Gene ID (NCBI) 10319
RRIDAB_2882532
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y6N6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for LAMC3-Specific antibody 67261-1-IgDownload protocol
WB protocol for LAMC3-Specific antibody 67261-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

bioRxiv

Laminin β4 is required for the development of human peripheral sensory neurons

Authors - Kenyi Saito-Diaz
View Article
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