验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human placenta tissue, LNCaP cells, pig rectum tissue, rat rectum tissue, HepG2 cells, JAR cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IF | See 1 publications below |
产品信息
67706-1-Ig targets LAMC1 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat, pig samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, rat, pig |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag14674 Product name: Recombinant human LAMC1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1260-1609 aa of BC015586 Sequence: AGDKAVEIYASVAQLSPLDSETLENEANNIKMEAENLEQLIDQKLKDYEDLREDMRGKELEVKNLLEKGKTEQQTADQLLARADAAKALAEEAAKKGRDTLQEANDILNNLKDFDRRVNDNKTAAEEALRKIPAINQTITEANEKTREAQQALGSAAADATEAKNKAHEAERIASAVQKNATSTKAEAERTFAEVTDLDNEVNNMLKQLQEAEKELKRKQDDADQDMMMAGMASQAAQEAEINARKAKNSVTSLLSIINDLLEQLGQLDTVDLNKLNEIEGTLNKAKDEMKVSDLDRKVSDLENEAKKQEAAIMDYNRDIEEIMKDIRNLEDIRKTLPSGCFNTPSIEKP 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | laminin, gamma 1 (formerly LAMB2) |
| 别名 | Laminin subunit gamma 1, 1G2G4, LAMB2, LAMC 1, Laminin subunit gamma-1 |
| 计算分子量 | 178 kDa |
| 观测分子量 | 200-220 kDa |
| GenBank蛋白编号 | BC015586 |
| 基因名称 | LAMC1 |
| Gene ID (NCBI) | 3915 |
| RRID | AB_2882897 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P11047 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for LAMC1 antibody 67706-1-Ig | Download protocol |
| WB protocol for LAMC1 antibody 67706-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neuroscience Comparative Proteomics of Rat Olfactory Bulb Reveal Insights into Susceptibility and Resiliency to Chronic-stress-induced Depression or Anxiety. | ||
Rheumatology (Oxford) The central helicase domain holds the major conformational epitopes of melanoma differentiation-associated gene 5 autoantibodies | ||
Exp Neurol LAMC1 attenuates neuronal apoptosis via FAK/PI3K/AKT signaling pathway after subarachnoid hemorrhage | ||
J Transl Med HIF-1α-mediated LAMC1 overexpression is an unfavorable predictor of prognosis for glioma patients: evidence from pan-cancer analysis and validation experiments |
