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验证数据展示

  • WB analysis using 31181-1-AP

    WB analysis using 31181-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31181-1-AP (LAMA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 31181-1-AP

    IHC staining of mouse skeletal muscle using 31181-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 31181-1-AP (LAMA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse skeletal muscle using 31181-1-AP

    IF Staining of mouse skeletal muscle using 31181-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skeletal muscle tissue using LAMA2 antibody (31181-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LAMA2 Polyclonal antibody

LAMA2 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 31181-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

LAMM, Laminin-4 subunit alpha, Laminin-2 subunit alpha, Laminin-12 subunit alpha, Laminin subunit alpha-2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman placenta tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IHC detected inmouse skeletal muscle tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skeletal muscle tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31181-1-AP targets LAMA2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag34494

Product name: Recombinant human LAMA2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2100-2250 aa of NM_000426

Sequence: IDKLKPIKELEDNLKKNISEIKELINQARKQANSIKVSVSSGGDCIRTYKPEIKKGSYNNIVVNVKTAVADNLLFYLGSAKFIDFLAIEMRKGKVSFLWDVGSGVGRVEYPDLTIDDSYWYRIVASRTGRNGTISVRALDG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 laminin, alpha 2
别名 LAMM, Laminin-4 subunit alpha, Laminin-2 subunit alpha, Laminin-12 subunit alpha, Laminin subunit alpha-2
计算分子量344 kDa
观测分子量344 kDa
GenBank蛋白编号NM_000426
基因名称 LAMA2
Gene ID (NCBI) 3908
RRIDAB_3669885
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP24043
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The laminin subunit alpha 2 (LAMA2) gene encodes an alpha 2 chain, which constitutes one of the subunits of laminin 2 (merosin) and laminin 4 (s-merosin). Basal laminae are structural components of the extracellular matrix that influence cell proliferation and differentiation,7 and laminin subunit alpha 2 (LAMA2) is the major component of the basal laminae-α subunit of laminin. (PMID: 30728939)

实验方案

Product Specific Protocols
IF protocol for LAMA2 antibody 31181-1-APDownload protocol
IHC protocol for LAMA2 antibody 31181-1-APDownload protocol
WB protocol for LAMA2 antibody 31181-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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