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验证数据展示

  • WB analysis using 28697-1-AP (same clone as 28697-1-PBS)

    WB analysis using 28697-1-AP (same clone as 28697-1-PBS)

    Various lysates were subjected to Tris-Acetate gel system followed by western blot with 28697-1-AP (LAMA1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28697-1-PBS in a different storage buffer formulation.

  • IF Staining of rat colon using 28697-1-AP (same clone as 28697-1-PBS)

    IF Staining of rat colon using 28697-1-AP (same clone as 28697-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat colon tissue using LAMA1 antibody (28697-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28697-1-PBS in a different storage buffer formulation.

LAMA1 Polyclonal antibody, PBS Only

LAMA1 Polyclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 28697-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF-P, Indirect ELISA

别名

Laminin 1 subunit alpha, laminin, alpha 1, LAMA, Laminin 3 subunit alpha, Laminin A chain

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

28697-1-PBS targets LAMA1 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF-P, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag29329

Product name: Recombinant human LAMA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1592-1778 aa of NM_005559

Sequence: SNLENTTKYLQESLLKENMQKDLGKIKLEGVAEETDNLQKKLTRMLASTQKVNRATERIFKESQDLAIAIERLQMSITEIMEKTTLNQTLDEDFLLPNSTLQNMQQNGTSLLEIMQIRDFTQLHQNATLELKAAEDLLSQIQENYQKPLEELEVLKEAASHVLSKHNNELKAAEALVREAEAKMQES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 laminin, alpha 1
别名 Laminin 1 subunit alpha, laminin, alpha 1, LAMA, Laminin 3 subunit alpha, Laminin A chain
计算分子量 337 kDa
观测分子量337 kDa
GenBank蛋白编号NM_005559
基因名称 LAMA1
Gene ID (NCBI) 284217
RRIDAB_3742299
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP25391
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LAMA1 is also known as laminin subunit α1. Laminins are a family of glycoproteins found in the extracellular matrix that comprises the basement membrane. Laminins have a heterotrimeric structure composed of an α, β, and γ chain. Numerous biological processes are known to be directed by them, including cell adhesion, mitogenesis, differentiation, and metastasis, all of which contribute to carcinogenesis. Tissue distribution of LAMA1 occurred mainly in early epithelial development and some adult epithelia. Recent reports have shown that laminin-1 acts as an efficient attachment protein for a large variety of cultured cell types in vitro. (PMID: 36824663)

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