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Host
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Species Reactivity
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验证数据展示

  • FC experiment of mouse splenocytes using 98167-1-RR (same clone as 98167-1-PBS)

    FC experiment of mouse splenocytes using 98167-1-RR (same clone as 98167-1-PBS)

    1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse LAG-3/CD223 Rabbit Recombinant Antibody (98167-1-RR, Clone: 241294E11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98167-1-PBS in a different storage buffer formulation.

  • FC experiment of mouse splenocytes using 98167-1-RR (same clone as 98167-1-PBS)

    FC experiment of mouse splenocytes using 98167-1-RR (same clone as 98167-1-PBS)

    1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse LAG-3/CD223 Rabbit Recombinant Antibody (98167-1-RR, Clone: 241294E11) (red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse LAG-3/CD223 Rabbit Recombinant Antibody (98167-1-RR, Clone:241294E11) (black, dashed) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98167-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98167-1-RR

    Affinity and Kinetic Characterization of 98167-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98167-1-RR against Mouse LAG-3/CD223 were performed. The affinity constant is 0.636 nM.

Anti-Mouse LAG-3/CD223 Rabbit Recombinant Antibody, PBS Only

LAG-3/CD223 Uni-rAbTM Recombinant Antibody for FC
Cat No. 98167-1-PBS

产品说明书

CloneNo. 241294E11

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

CD223, LAG-3, Ly66, Lag3, LAG 3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

98167-1-PBS targets LAG-3/CD223 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原 Fusion Protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 lymphocyte-activation gene 3
别名 CD223, LAG-3, Ly66, Lag3, LAG 3
计算分子量57kDa
GenBank蛋白编号NM_008479.2
基因名称 CD223
Gene ID (NCBI) 16768
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ61790
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Lymphocyte activation gene 3 protein (LAG-3), also known as CD223, is a type I transmembrane protein belonging to the immunoglobulin superfamily (PMID: 1692078). It is a CD4-related molecule that binds MHC class II with high affinity (PMID: 1692078; 9159144). LAG-3 is expressed on activated T and NK cells. Besides, natural CD4(+)CD25(+) Tregs express LAG-3 upon activation (PMID: 15485628). LAG-3 negatively regulates T-cell function and homeostasis, and contributes to the suppressor activity of Tregs (PMID: 14644131; 15485628).