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验证数据展示

  • WB analysis using 16616-1-AP (same clone as 16616-1-PBS)

    WB analysis using 16616-1-AP (same clone as 16616-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16616-1-AP (LAG-3/CD223 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16616-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 16616-1-AP (same clone as 16616-1-PBS)

    IHC staining of human tonsillitis using 16616-1-AP (same clone as 16616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16616-1-AP (LAG-3/CD223 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16616-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 16616-1-AP (same clone as 16616-1-PBS)

    IHC staining of human tonsillitis using 16616-1-AP (same clone as 16616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16616-1-AP (LAG-3/CD223 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16616-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse liver using 16616-1-AP (same clone as 16616-1-PBS)

    IP experiment of mouse liver using 16616-1-AP (same clone as 16616-1-PBS)

    IP result of anti-LAG-3 (IP:16616-1-AP, 4ug; Detection:16616-1-AP 1:500) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 16616-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 16616-1-AP (same clone as 16616-1-PBS)

    IF Staining of human tonsillitis using 16616-1-AP (same clone as 16616-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LAG-3 antibody (16616-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16616-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 16616-1-AP (same clone as 16616-1-PBS)

    IF Staining of human tonsillitis using 16616-1-AP (same clone as 16616-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using LAG-3 antibody (16616-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16616-1-PBS in a different storage buffer formulation.

LAG-3/CD223 Polyclonal antibody, PBS Only

LAG-3/CD223 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Cat No. 16616-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, IP, ELISA

别名

CD223, LAG3, FDC, LAG 3, LAG-3

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

16616-1-PBS targets LAG-3/CD223 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9909

Product name: Recombinant human LAG3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 27-360 aa of BC052589

Sequence: AEVPVVWAQEGAPAQLPCSPTIPLQDLSLLRRAGVTWQHQPDSGPPAAAPGHPLAPGPHPAAPSSWGPRPRRYTVLSVGPGGLRSGRLPLQPRVQLDERGRQRGDFSLWLRPARRADAGEYRAAVHLRDRALSCRLRLRLGQASMTASPPGSLRASDWVILNCSFSRPDRPASVHWFRNRGQGRVPVRESPHHHLAESFLFLPQVSPMDSGPWGCILTYRDGFNVSIMYNLTVLGLEPPTPLTVYAGAGSRVGLPCRLPAGVGTRSFLTAKWTPPGGGPDLLVTGDNGDFTLRLEDVSQAQAGTYTCHIHLQEQQLNATVTLAIITGQPQVGKE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lymphocyte-activation gene 3
别名 CD223, LAG3, FDC, LAG 3, LAG-3
计算分子量 525 aa, 57 kDa
观测分子量60-70 kDa
GenBank蛋白编号BC052589
基因名称 LAG-3
Gene ID (NCBI) 3902
RRIDAB_2133350
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP18627
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

LAG-3, also known as CD223, is an immune checkpoint molecule that regulates both T-cell activation and homeostasis. LAG-3 is expressed on activated T cells, NK cells, regulatory T cells, and plasmacytoid dendritic cells. It is a CD4-related molecule that binds MHC class II. LAG-3 plays an important role in modulating T cell expansion and function, and blockade of LAG-3 with monoclonal antibodies can augment T cell function. (PMID: 15634887; 21086108; 28783703)

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