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LAD1 Polyclonal antibody

LAD1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 16136-1-AP

Print datasheet

Synonyms

LAD, Lad 1, LAD1, LadA, ladinin 1, Linear IgA disease antigen

验证数据展示

  • WB analysis using 16136-1-AP

    WB analysis using 16136-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 16136-1-AP (LAD1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 16136-1-AP

    IHC staining of human colon cancer using 16136-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16136-1-AP (LAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16136-1-AP targets LAD1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen LAD1 fusion protein Ag9159 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ladinin 1
Synonyms LAD, Lad 1, LAD1, LadA, ladinin 1, Linear IgA disease antigen
Calculated Molecular Weight 517 aa, 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC009742
Gene Symbol LAD1
Gene ID (NCBI) 3898
RRIDAB_2081705
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00515
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Ladinin-1, also named as LADA, or LAD1, is an anchoring filament protein which is a component of the basement membrane zone. It is a novel component of the basement membranes and may function in contributing to the stability of the association of the epithelial layers with the underlying mesenchyme. Human ladinin is an autoantigen associated with linear IgA disease. It is a secreted protein. Immunohistochemical staining of 16136-1-AP shows strong extracellular matrixc positivity in human testis. The predicted MW of this protein is 57 kDa.

实验方案

Product Specific Protocols
WB protocol for LAD1 antibody 16136-1-APDownload protocol
IHC protocol for LAD1 antibody 16136-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF

J Transl Med

LAD1 promotes malignant progression by diminishing ubiquitin-dependent degradation of vimentin in gastric cancer

Authors - Yingming Jiang
View Article