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验证数据展示

  • WB analysis using 28112-1-AP (same clone as 28112-1-PBS)

    WB analysis using 28112-1-AP (same clone as 28112-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28112-1-AP (L3MBTL3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28112-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 28112-1-AP (same clone as 28112-1-PBS)

    IHC staining of mouse cerebellum using 28112-1-AP (same clone as 28112-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28112-1-AP (L3MBTL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28112-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 28112-1-AP (same clone as 28112-1-PBS)

    IF Staining of U2OS using 28112-1-AP (same clone as 28112-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using L3MBTL3 antibody (28112-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28112-1-PBS in a different storage buffer formulation.

L3MBTL3 Polyclonal antibody, PBS Only

L3MBTL3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28112-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

28112-1-PBS targets L3MBTL3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag27136

Product name: Recombinant human L3MBTL3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 524-646 aa of BC060845

Sequence: LMEASEHGGCSTPGCKGIGHFKRARHLGPHSAANCPYSEINLNKDRIFPDRLSGEMPPASPSFPRNKRTDANESSSSPEIRDQHADDVKEDFEERTESEMRTSHEARGAREEPTVQQAQRRSA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 l(3)mbt-like 3 (Drosophila)
别名
计算分子量 88 aa, 3 kDa
观测分子量88 kDa
GenBank蛋白编号BC060845
基因名称 L3MBTL3
Gene ID (NCBI) 84456
RRIDAB_2918148
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96JM7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

L3MBTL3, also named as MBT1 and KIAA1798, belongs to the Malignant brain tumor (MBT) family. L3MBTL3 can bind to methyl lysine in histone, further affecting the transcription and its abnormal regulation associated with many diseases (PMID: 34373992). Additionally, researches have shown that L3MBTL3 plays a role in meduloblastoma formation and normal hematopoiesis in humans (PMID: 26317848). L3MBTL3 has 2 isoforms with the molecular mass of 86 and 88 kDa.

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