验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, PC-3 cells, mouse kidney tissue, mouse liver tissue, rat kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33146-1-AP targets L3HYPDH in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag37121 Product name: Recombinant human C14orf149 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 195-354 aa of BC012131 Sequence: VTAEKLGLDICSAKTRDLVDAASAVTEAVKAQFKINHPDSEDLAFLYGTILTDGKDAYTKEPTTNICVFADEQVDRSPTGSGVTARIALQYHKGLLELNQMRAFKSSATGSVFTGKAVREAKCGDFKAVIVEVSGQAHYTGTASFIIEDDDPLRDGFLLK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | chromosome 14 open reading frame 149 |
| 别名 | C14orf149, EC:4.2.1.77, Trans-3-hydroxy-L-proline dehydratase, Trans-L-3-hydroxyproline dehydratase |
| 计算分子量 | 38 kDa |
| 观测分子量 | 40 kDa |
| GenBank蛋白编号 | BC012131 |
| 基因名称 | C14orf149 |
| Gene ID (NCBI) | 112849 |
| RRID | AB_3742871 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q96EM0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Trans-3-hydroxy-L-proline dehydratase (L3HYPDH) is an enzyme that catalyzes the conversion of trans-3-hydroxy-L-proline to delta(1)-pyrroline-2-carboxylate. This enzyme is involved in the degradation of dietary proteins containing trans-3-hydroxy-L-proline, such as collagen IV. Additionally, L3HYPDH has been identified as an interferon-stimulated gene (ISG) with antiviral activity, particularly against viruses like EV71 (PMID: 35891782). It also plays a role in the genetic regulation of working memory and has been implicated in the mitochondrial pathway associated with autism spectrum disorder (PMID: 32490597).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for L3HYPDH antibody 33146-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
