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验证数据展示

  • IF Staining of A431 using 55208-1-AP

    IF Staining of A431 using 55208-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A431 cells using 55208-1-AP (L-Plastin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 55208-1-AP

    WB analysis using 55208-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55208-1-AP (L-Plastin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 55208-1-AP

    IHC staining of human colon cancer using 55208-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55208-1-AP (L-Plastin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

L-Plastin Polyclonal antibody

L-Plastin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 55208-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

LCP1, L PLASTIN, LCP 1, LCP-1, LPL

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, K-562 cells, Ramos cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55208-1-AP targets L-Plastin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 lymphocyte cytosolic protein 1 (L-plastin)
别名 LCP1, L PLASTIN, LCP 1, LCP-1, LPL
计算分子量 70 kDa
观测分子量 64-70 kDa
GenBank蛋白编号NM_002298
基因名称 L-Plastin
Gene ID (NCBI) 3936
RRIDAB_2881288
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13796
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LCP1, also known as L-Plastin, is a member of the Plastin gene family. The family members include L-Plastin (LCP1), T-Plastin (PLS3), and I-Plastin (PLS1), which can regulate the dynamic reorganization of the cytoskeleton by binding and cross-linking actin filaments. In lymphocytes, LCP1 can regulate immune responses, especially during inflammation and infection. It acts as a key regulator of T-cell activation by responding to co-stimulatory signals through TCR/CD3 and CD2 or CD28, and regulates the cell-surface expression of IL2RA/CD25 and CD69 (PMID: 17294403).

实验方案

Product Specific Protocols
WB protocol for L-Plastin antibody 55208-1-APDownload protocol
IHC protocol for L-Plastin antibody 55208-1-APDownload protocol
IF protocol for L-Plastin antibody 55208-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols