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验证数据展示

  • WB analysis of human plasma using 84175-5-RR

    WB analysis of human plasma using 84175-5-RR

    Human plasma was subjected to SDS PAGE followed by western blot with 84175-5-RR (Kininogen-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HT-29 using 84175-5-RR

    FC experiment of HT-29 using 84175-5-RR

    1x10^6 HT-29 cells were intracellularly stained with 0.25 ug Kininogen-1 Recombinant antibody (84175-5-RR, Clone:241397C11) (red), or 0.25 ug Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84175-5-RR

    Affinity and Kinetic Characterization of 84175-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84175-5-RR against Human Kininogen-1 were performed. The affinity constant is 0.182 nM.

Kininogen 1 Recombinant antibody

Kininogen 1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84175-5-RR

产品说明书

CloneNo. 241397C11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

KNG1, 241397C11, Alpha-2-thiol proteinase inhibitor, BDK, Bradykinin

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma
Positive FC (Intra) detected inHT-29 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84175-5-RR targets Kininogen 1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 kininogen 1
别名 KNG1, 241397C11, Alpha-2-thiol proteinase inhibitor, BDK, Bradykinin
计算分子量72kDa
观测分子量 48 kDa
GenBank蛋白编号NM_001102416.3
基因名称 Kininogen 1
Gene ID (NCBI) 3827
RRIDAB_3671732
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP01042-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kininogens are inhibitors of thiol proteases. Kininogen 1 plays important role in Kinin-kallikrein system. This KNG1 gene contains 10 exons and encodes two proteins through alternative splicing: high molecular weight kininogen (HMWK) (72kDa) and low molecular weight kininogen (LMWK) (48kDa) (PMID: 37442062). HMWK is produced by the liver together with prekallikrein. It acts mainly as a cofactor on coagulation and inflammation, and has no intrinsic catalytic activity. LMWK is produced locally by numerous tissues, and secreted together with tissue kallikrein.

实验方案

Product Specific Protocols
WB protocol for Kininogen 1 antibody 84175-5-RRDownload protocol
FC protocol for Kininogen 1 antibody 84175-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols