验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A549 cells, HepG2 cells, rat liver tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11796-1-AP targets KYNU in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag2365 Product name: Recombinant human KYNU protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-307 aa of BC000879 Sequence: MEPSSLELPADTVQRIAAELKCHPTDERVALHLDEEDKLRHFRECFYIPKIQDLPPVDLSLVNKDENAIYFLGNSLGLQPKMVKTYLEEELDKWAKIAAYGHEVGKRPWITGDESIVGLMKDIVGANEKEIALMNALTVNLHLLMLSFFKPTPKRYKILLEAKAFPSDHYAIESQLQLHGLNIEESMRMIKPREGEETLRIEDILEVIEKEGDSIAVILFSGVHFYTGQHFNIPAITKAGQAKGCYVGFDLAHAVGNVELYLHDWGVDFACWCSYKYLNAGAGGIAGAFIHEKHAHTIKPARSEFFN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | kynureninase (L-kynurenine hydrolase) |
| 别名 | EC:3.7.1.3, Kynureninase, L kynurenine hydrolase, L-kynurenine hydrolase |
| 计算分子量 | 465aa,52 kDa; 307aa,35 kDa |
| 观测分子量 | 45-52 kDa |
| GenBank蛋白编号 | BC000879 |
| 基因名称 | KYNU |
| Gene ID (NCBI) | 8942 |
| RRID | AB_2133050 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16719 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KYNU(kynureninase) is also named as L-kynurenine hydrolase and belongs to the kynureninase family. It is a pyridoxal-5'-phosphate-(pyridoxal-P)-depe11dent enzyme that catalyses the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively(PMID:8706755). In SDS-PAGE, it can detect a 48 kDa band lower than the preidicted molecular weight of 52 kDa due to limited proteolysis of the enzyme which might occur during handling of the protein(PMID:8706755). This protein is a 95-kD homodimer predominantly located in the cytoplasm(PMID:9180257).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for KYNU antibody 11796-1-AP | Download protocol |
| IHC protocol for KYNU antibody 11796-1-AP | Download protocol |
| WB protocol for KYNU antibody 11796-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Hazard Mater Particulate matter-induced metabolic recoding of epigenetics in macrophages drives pathogenesis of chronic obstructive pulmonary disease | ||
Virol Sin Establishment and characterization of a new cell culture system for hepatitis B virus replication and infection. | ||
Chem Res Toxicol Tryptophan Pathway-Targeted Metabolomics Study on the Mechanism and Intervention of Cisplatin-Induced Acute Kidney Injury in Rats. | ||
Clin Exp Dermatol Downregulation of kynureninase restrains cutaneous squamous cell carcinoma proliferation and represses the PI3K/AKT pathway.
| ||
Adv Sci (Weinh) Tryptophan Metabolism Acts as a New Anti-Ferroptotic Pathway to Mediate Tumor Growth
| ||
Oncogene Glutathione peroxidase 2 knockdown suppresses gastric cancer progression and metastasis via regulation of kynurenine metabolism |
