验证数据展示

WB analysis using 19841-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 19841-1-AP (KTN1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IHC staining of human ovary tumor using 19841-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 19841-1-AP (KTN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with KTN1 antibody included
IP experiment of Jurkat using 19841-1-AP
IP result of anti-KTN1 (IP:19841-1-AP, 4ug; Detection:19841-1-AP 1:500) with Jurkat cells lysate 1200ug.
WB analysis of A549 using 19841-1-AP
WB result of KTN1 antibody (19841-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KTN1 transfected A549 cells.
IHC staining of human bowen disease using 19841-1-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 19841-1-AP (KTN1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with KTN1 antibody included
IHC staining of human colon using 19841-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 19841-1-AP (KTN1 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with KTN1 antibody included
IF Staining of HepG2 using 19841-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KTN1 antibody (19841-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 19841-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KTN1 antibody (19841-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Featured Product
KD/KO验证

KTN1 Polyclonal antibody

KTN1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 19841-1-AP

产品说明书

引用文献(9)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

别名

KTN 1, KIAA0004, CG-1 antigen

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

规格:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (9)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	HeLa cells, A549 cells, Jurkat cells, K-562 cells, PC-3 cells, SKOV-3 cells
Positive IP detected in	Jurkat cells
Positive IHC detected in	human ovary tumor tissue, human bowen disease, human colon tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Planning an IHC experiment? We recommend our IHCeasy KTN1 Ready-To-Use IHC Kit. KTN1 primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 3 publications below
WB	See 6 publications below
IHC	See 1 publications below
IF	See 3 publications below
CoIP	See 1 publications below

产品信息

19841-1-AP targets KTN1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用	WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用	WB, IHC, IF, CoIP
经测试反应性	human, mouse
文献引用反应性	human, mouse, monkey
免疫原	KTN1 fusion protein Ag13854 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	kinectin 1 (kinesin receptor)
别名	KTN 1, KIAA0004, CG-1 antigen
计算分子量	1357 aa, 156 kDa
观测分子量	156 kDa
GenBank蛋白编号	BC117132
基因名称	KTN1
Gene ID (NCBI)	3895
RRID	AB_10642436
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	Q86UP2
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

KTN1/Kinectin is an integral endoplasmic reticulum (ER) membrane protein which acts as dynamic anchor to facilitate ER membrane extension along microtubules. It has been found to bind kinesin, Rho GTPase, and translation elongation factor-1delta and gets involved in organelle motility and protein synthesis.

实验方案

Product Specific Protocols
WB protocol for KTN1 antibody 19841-1-AP	Download protocol
IHC protocol for KTN1 antibody 19841-1-AP	Download protocol
IF protocol for KTN1 antibody 19841-1-AP	Download protocol
IP protocol for KTN1 antibody 19841-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human,monkey	WB	Nature ER proteins decipher the tubulin code to regulate organelle distribution Authors - Pengli Zheng KO Validated View Article
human,mouse	WB	Sci Transl Med CPEB alteration and aberrant transcriptome-polyadenylation lead to a treatable SLC19A3 deficiency in Huntington's disease. Authors - Sara Picó View Article
human	IF	Cell Res DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling. Authors - Pengli Zheng View Article
	IF	EMBO Rep Mobile late endosomes modulate peripheral endoplasmic reticulum network architecture. Authors - Menno Spits View Article
human	WB, IHC	Clin Sci (Lond) The long noncoding RNA KTN1-AS1 promotes bladder cancer tumorigenesis via KTN1 cis-activation and the consequent initiation of Rho GTPase-mediated signaling. Authors - Xueying Hu View Article
monkey	WB	Curr Biol ATL3 Is a Tubular ER-Phagy Receptor for GABARAP-Mediated Selective Autophagy. Authors - Qingzhou Chen View Article

验证数据展示

WB analysis using 19841-1-AP

IHC staining of human ovary tumor using 19841-1-AP

IP experiment of Jurkat using 19841-1-AP

WB analysis of A549 using 19841-1-AP

IHC staining of human bowen disease using 19841-1-AP

IHC staining of human colon using 19841-1-AP

IF Staining of HepG2 using 19841-1-AP

IF Staining of HepG2 using 19841-1-AP

KTN1 Polyclonal antibody

KTN1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

ER proteins decipher the tubulin code to regulate organelle distribution

CPEB alteration and aberrant transcriptome-polyadenylation lead to a treatable SLC19A3 deficiency in Huntington's disease.

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Mobile late endosomes modulate peripheral endoplasmic reticulum network architecture.

The long noncoding RNA KTN1-AS1 promotes bladder cancer tumorigenesis via KTN1 cis-activation and the consequent initiation of Rho GTPase-mediated signaling.

ATL3 Is a Tubular ER-Phagy Receptor for GABARAP-Mediated Selective Autophagy.