- Featured Product
- KD/KO Validated
KRT81 Polyclonal antibody
KRT81 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, mouse thymus tissue, human spleen tissue, HEK-293 cells, MCF-7 cells, mouse pancreas tissue |
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 2 publications below |
WB | See 2 publications below |
IHC | See 1 publications below |
产品信息
11342-1-AP targets KRT81 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IHC |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | KRT81 fusion protein Ag1890 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | keratin 81 |
Synonyms | ghHb1, ghHkb1, Hair keratin K2.9, Hb 1, HB1, hHAKB2 1, K81, keratin 81, Keratin, hair, basic, 1, Keratin, type II cuticular Hb1, KRT81, KRTHB1, MLN 137, MLN137, Type II hair keratin Hb1, Type II keratin Kb21 |
Calculated Molecular Weight | 55 kDa |
Observed Molecular Weight | 54 kDa |
GenBank Accession Number | BC021241 |
Gene Symbol | KRT81 |
Gene ID (NCBI) | 3887 |
RRID | AB_2132771 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q14533 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for KRT81 antibody 11342-1-AP | Download protocol |
IHC protocol for KRT81 antibody 11342-1-AP | Download protocol |
IF protocol for KRT81 antibody 11342-1-AP | Download protocol |
IP protocol for KRT81 antibody 11342-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Clin Cancer Res A Preclinical Trial and Molecularly Annotated Patient Cohort Identify Predictive Biomarkers in Homologous Recombination-deficient Pancreatic Cancer. | ||
Oncol Rep Hair keratin KRT81 is expressed in normal and breast cancer cells and contributes to their invasiveness. | ||
Heliyon Single-cell data analysis of malignant epithelial cell heterogeneity in lung adenocarcinoma for patient classification and prognosis prediction
| ||
Adv Sci (Weinh) Ectopic Expression of a Truncated Isoform of Hair Keratin 81 in Breast Cancer Alters Biophysical Characteristics to Promote Metastatic Propensity
|