• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

KRT33B Polyclonal antibody

KRT33B Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 11048-1-AP

Print datasheet

Synonyms

Ha 3II, HA3II, Hair keratin, type I Ha3 II, hHa3 II, HKA3B, K33B, keratin 33B, KRT33B, KRTHA3A, KRTHA3B

验证数据展示

  • WB analysis of MCF-7 using 11048-1-AP

    WB analysis of MCF-7 using 11048-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 11048-1-AP (KRT33B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 11048-1-AP

    IHC staining of human skin cancer using 11048-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 11048-1-AP (KRT33B antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT33B antibody included
  • IHC staining of human skin cancer using 11048-1-AP

    IHC staining of human skin cancer using 11048-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 11048-1-AP (KRT33B antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT33B antibody included
  • IF Staining of MCF-7 using 11048-1-AP

    IF Staining of MCF-7 using 11048-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 11048-1-AP (KRT33B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inMCF-7 cells, human brain tissue
Positive IHC detected inhuman skin cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy KRT33B Ready-To-Use IHC Kit. KRT33B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

11048-1-AP targets KRT33B in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen KRT33B fusion protein Ag1522 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 33B
Synonyms Ha 3II, HA3II, Hair keratin, type I Ha3 II, hHa3 II, HKA3B, K33B, keratin 33B, KRT33B, KRTHA3A, KRTHA3B
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC009971
Gene Symbol KRT33B
Gene ID (NCBI) 3884
RRIDAB_2133874
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14525
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 33 belongs to type I keratin and is found in hair and nails. There are two isoforms of this protein, encoded by two separate genes, KRTHA3A and KRTHA3B.

实验方案

Product Specific Protocols
WB protocol for KRT33B antibody 11048-1-APDownload protocol
IHC protocol for KRT33B antibody 11048-1-APDownload protocol
IF protocol for KRT33B antibody 11048-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Asian J Androl

PMID: 24994782

Authors - Yan-Min Ma
View Article