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验证数据展示

  • WB analysis of BxPC-3 using 24049-1-AP

    WB analysis of BxPC-3 using 24049-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 24049-1-AP (KRT23 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 24049-1-AP

    IHC staining of human colon cancer using 24049-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT23 antibody included
  • IHC staining of human colon cancer using 24049-1-AP

    IHC staining of human colon cancer using 24049-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT23 antibody included
  • IHC staining of human bowen disease using 24049-1-AP

    IHC staining of human bowen disease using 24049-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT23 antibody included
  • IHC staining of human bowen disease using 24049-1-AP

    IHC staining of human bowen disease using 24049-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 24049-1-AP (KRT23 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT23 antibody included

Cytokeratin 23 Polyclonal antibody

Cytokeratin 23 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24049-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA and More (1)

别名

KRT23, CK 23, CK23, CK-23, Cytokeratin-23

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inBxPC-3 cells, HepG2 cells, HeLa cells
Positive IHC detected inhuman colon cancer tissue, human skin cancer tissue, human placenta tissue, human liver cancer tissue, rat colon tissue, mouse colon tissue, human bowen disease
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy KRT23 Ready-To-Use IHC Kit. KRT23 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

24049-1-AP targets Cytokeratin 23 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag21129

Product name: Recombinant human KRT23 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1-422 aa of BC028356

Sequence: MNSGHSFSQTPSASFHGAGGGWGRPRSFPRAPTVHGGAGGARISLSFTTRSCPPPGGSWGSGRSSPLLGGNGKATMQNLNDRLASYVEKVRALEEANMKLESRILKWHQQRDPGSKKDYSQYEENITHLQEQIVDGKMTNAQIILLIDNARMAVDDFNLKYENEHSFKKDLEIEVEGLRRTLDNLTIVTTDLEQEVEGMRKELILMKKHHEQEMEKHHVPSDFNVNVKVDTGPREDLIKVLEDMRQEYELIIKKKHRDLDTWYKEQSAAMSQEAASPATVQSRQGDIHELKRTFQALEIDLQTQYSTKSALENMLSETQSRYSCKLQDMQEIISHYEEELTQLRHELERQNNEYQVLLGIKTHLEKEITTYRRLLEGESEGTREESKSSMKVFATPKIKAITQETINGRLVLCQVNEIQKHA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 23 (histone deacetylase inducible)
别名 KRT23, CK 23, CK23, CK-23, Cytokeratin-23
计算分子量 422 aa, 48 kDa
观测分子量 48 kDa
GenBank蛋白编号BC028356
基因名称 KRT23
Gene ID (NCBI) 25984
RRIDAB_2879416
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9C075
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT23 belongs to type I keratins.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 23 antibody 24049-1-APDownload protocol
IHC protocol for Cytokeratin 23 antibody 24049-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Cell Rep

Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed embryonic stem cell-based differentiation model.

Authors - Masatoshi Ohgushi
View Article