KRT23 Polyclonal antibody
KRT23 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse
Applications
IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | BxPC-3 cells, HepG2 cells, HeLa cells |
| Positive IHC detected in | human colon cancer tissue, human skin cancer tissue, human placenta tissue, human liver cancer tissue, rat colon tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy KRT23 Ready-To-Use IHC Kit. KRT23 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
24049-1-AP targets KRT23 in WB, IF, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Applications | IHC, WB, ELISA |
| Cited Applications | IF |
| Tested Reactivity | human, rat, mouse |
| Cited Reactivity | human |
| Immunogen | KRT23 fusion protein Ag21129 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | keratin 23 (histone deacetylase inducible) |
| Synonyms | CK 23, CK23, Cytokeratin 23, HAIK1, K23, Keratin 23, KRT23 |
| Calculated Molecular Weight | 422 aa, 48 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC028356 |
| Gene Symbol | KRT23 |
| Gene ID (NCBI) | 25984 |
| RRID | AB_2879416 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9C075 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT23 belongs to type I keratins.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KRT23 antibody 24049-1-AP | Download protocol |
| IHC protocol for KRT23 antibody 24049-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed embryonic stem cell-based differentiation model. |
