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验证数据展示

  • WB analysis of HeLa using 22230-1-AP

    WB analysis of HeLa using 22230-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22230-1-AP (Cytokeratin 17 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 22230-1-AP

    IF Staining of HeLa using 22230-1-AP

    Immunofluorescent analysis of HeLa cells using 22230-1-AP (Cytokeratin 17 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human cervical cancer using 22230-1-AP

    IHC staining of human cervical cancer using 22230-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 22230-1-AP (Cytokeratin 17 antibody at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with KRT17 antibody included
  • IHC staining of human cervical cancer using 22230-1-AP

    IHC staining of human cervical cancer using 22230-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 22230-1-AP (Cytokeratin 17 antibody at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with KRT17 antibody included
  • IHC staining of human bowen disease using 22230-1-AP

    IHC staining of human bowen disease using 22230-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 22230-1-AP (Cytokeratin 17 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT17 antibody included
  • IHC staining of human bowen disease using 22230-1-AP

    IHC staining of human bowen disease using 22230-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 22230-1-AP (Cytokeratin 17 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT17 antibody included

Cytokeratin 17 Polyclonal antibody

Cytokeratin 17 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 22230-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

KRT17, Keratin-17, keratin 17, Cytokeratin-17, Cytokeratin,Keratin

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells
Positive IHC detected inhuman bowen disease, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy KRT17 Ready-To-Use IHC Kit. KRT17 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22230-1-AP targets Cytokeratin 17 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag17575

Product name: Recombinant human KRT17 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-87 aa of BC000159

Sequence: MTTSIRQFTSSSSIKGSSGLGGGSSRTSCRLSGGLGAGSCRLGSAGGLGSTLGGSSYSSCYSFGSGGGYGSSFGGVDGLLAGGEKAT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 17
别名 KRT17, Keratin-17, keratin 17, Cytokeratin-17, Cytokeratin,Keratin
计算分子量 432 aa, 48 kDa
观测分子量 48 kDa
GenBank蛋白编号BC000159
基因名称 Cytokeratin 17
Gene ID (NCBI) 3872
RRIDAB_2879040
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ04695
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 17 is a type I cytokeratin. It is found in nail beds, hair follicles, sebaceous glands, and other epidermal appendages. It is used as a marker for trauma.

实验方案

Product Specific Protocols
IF protocol for Cytokeratin 17 antibody 22230-1-APDownload protocol
IHC protocol for Cytokeratin 17 antibody 22230-1-APDownload protocol
WB protocol for Cytokeratin 17 antibody 22230-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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