验证数据展示
经过测试的应用
Positive WB detected in | mouse eye tissue |
Positive IHC detected in | mouse eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 6 publications below |
产品信息
24789-1-AP targets Cytokeratin 12 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | IF |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse |
免疫原 | Cytokeratin 12 fusion protein Ag20312 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | keratin 12 |
别名 | KRT12, CK 12, CK-12, Cytokeratin-12, keratin 12 |
计算分子量 | 494 aa, 54 kDa |
观测分子量 | 50-55 kDa |
GenBank蛋白编号 | BC156641 |
基因名称 | KRT12 |
Gene ID (NCBI) | 3859 |
RRID | AB_2879726 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q99456 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for Cytokeratin 12 antibody 24789-1-AP | Download protocol |
IHC protocol for Cytokeratin 12 antibody 24789-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Invest Ophthalmol Vis Sci Wounding Induces Facultative Opn5-Dependent Circadian Photoreception in the Murine Cornea. | ||
Exp Eye Res Low-dose repeated exposure to chemical surfactant impairs corneal epithelium: When personal cleaning products entering the eye. | ||
Int Ophthalmol ZD6474 attenuates TGF-β1-induced fibrosis in human Tenon fibroblasts and inhibits neovascularization via AKT-mTOR signaling pathway | ||
J Ocul Pharmacol Ther ZD6474 Attenuates Fibrosis and Inhibits Neovascularization in Human Pterygium by Suppressing AKT-mTOR Signaling Pathway | ||
Transl Vis Sci Technol Unraveling the Intricate Network of lncRNAs in Corneal Epithelial Wound Healing: Insights Into the Regulatory Role of linc17500 |