验证数据展示
经过测试的应用
| Positive WB detected in | mouse eye tissue |
| Positive IHC detected in | mouse eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy KRT12 Ready-To-Use IHC Kit. KRT12 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 7 publications below |
产品信息
24789-1-AP targets Cytokeratin 12 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag20312 Product name: Recombinant human KRT12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 369-494 aa of BC156641 Sequence: LEDSLAEAEGDYCAQLSQVQQLISNLEAQLLQVRADAERQNVDHQRLLNVKARLELEIETYRRLLDGEAQGDGLEESLFVTDSKSQAQSTDSSKDPTKTRKIKTVVQEMVNGEVVSSQVQEIEELM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | keratin 12 |
| 别名 | KRT12, CK 12, CK-12, Cytokeratin-12, keratin 12 |
| 计算分子量 | 494 aa, 54 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | BC156641 |
| 基因名称 | KRT12 |
| Gene ID (NCBI) | 3859 |
| RRID | AB_2879726 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q99456 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cytokeratin 12 antibody 24789-1-AP | Download protocol |
| IHC protocol for Cytokeratin 12 antibody 24789-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Invest Ophthalmol Vis Sci The Spatial Transcriptomic Atlas of Human Limbus and Vital Niche Microenvironment Regulating the Fate of Limbal Epithelial Stem Cells | ||
Invest Ophthalmol Vis Sci Wounding Induces Facultative Opn5-Dependent Circadian Photoreception in the Murine Cornea. | ||
Exp Eye Res Low-dose repeated exposure to chemical surfactant impairs corneal epithelium: When personal cleaning products entering the eye. | ||
Int Ophthalmol ZD6474 attenuates TGF-β1-induced fibrosis in human Tenon fibroblasts and inhibits neovascularization via AKT-mTOR signaling pathway | ||
J Ocul Pharmacol Ther ZD6474 Attenuates Fibrosis and Inhibits Neovascularization in Human Pterygium by Suppressing AKT-mTOR Signaling Pathway |