Cytokeratin 12 Polyclonal antibody

Cytokeratin 12 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24789-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA and More (1)

KRT12, CK 12, CK-12, Cytokeratin-12, keratin 12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse eye tissue
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy KRT12 Ready-To-Use IHC Kit. KRT12 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 6 publications below

产品信息

24789-1-AP targets Cytokeratin 12 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 Cytokeratin 12 fusion protein Ag20312 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 12
别名 KRT12, CK 12, CK-12, Cytokeratin-12, keratin 12
计算分子量 494 aa, 54 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC156641
基因名称 KRT12
Gene ID (NCBI) 3859
RRIDAB_2879726
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99456
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 12 antibody 24789-1-APDownload protocol
IHC protocol for Cytokeratin 12 antibody 24789-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

EBioMedicine

Cisd2 plays an essential role in corneal epithelial regeneration.

Authors - Chi-Chin Sun
mouseIF

Invest Ophthalmol Vis Sci

Wounding Induces Facultative Opn5-Dependent Circadian Photoreception in the Murine Cornea.

Authors - Nicolás M Díaz
mouseIF

Exp Eye Res

Low-dose repeated exposure to chemical surfactant impairs corneal epithelium: When personal cleaning products entering the eye.

Authors - Jie Wu
humanIF

Int Ophthalmol

ZD6474 attenuates TGF-β1-induced fibrosis in human Tenon fibroblasts and inhibits neovascularization via AKT-mTOR signaling pathway

Authors - Wenting Liu
humanIF

J Ocul Pharmacol Ther

ZD6474 Attenuates Fibrosis and Inhibits Neovascularization in Human Pterygium by Suppressing AKT-mTOR Signaling Pathway

Authors - Wenting Liu
mouseIF

Transl Vis Sci Technol

Unraveling the Intricate Network of lncRNAs in Corneal Epithelial Wound Healing: Insights Into the Regulatory Role of linc17500

Authors - Qiongjie Cao
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