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验证数据展示

  • IHC staining of human skin cancer using 16855-1-AP

    IHC staining of human skin cancer using 16855-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT10 antibody included
  • IHC staining of human skin cancer using 16855-1-AP

    IHC staining of human skin cancer using 16855-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT10 antibody included
  • WB analysis using 16855-1-AP

    WB analysis using 16855-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HaCaT using 16855-1-AP

    IF Staining of HaCaT using 16855-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using Cytokeratin 10-specific antibody (16855-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Cytokeratin 10-specific Polyclonal antibody

Cytokeratin 10-specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 16855-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

KRT10, Cytokeratin-10, Cytokeratin,Keratin, Cytokeratin 10, CK-10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse skin tissue, rat skin tissue
Positive IHC detected inhuman skin cancer tissue, human breast cancer tissue, mouse skin tissue, rat skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHaCaT cells
Planning an IHC experiment? We recommend our IHCeasy KRT10 Ready-To-Use IHC Kit. KRT10 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16855-1-AP targets Cytokeratin 10-specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 10
别名 KRT10, Cytokeratin-10, Cytokeratin,Keratin, Cytokeratin 10, CK-10
计算分子量 59 kDa
观测分子量59 kDa
GenBank蛋白编号NM_000421
基因名称 Cytokeratin 10
Gene ID (NCBI) 3858
RRIDAB_10792092
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13645
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. As a type I keratin, keratin 10 is a suprabasal marker of differentiation in stratified squamous epithelia. This antibody is specifically against KRT10.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 10-specific antibody 16855-1-APDownload protocol
IHC protocol for Cytokeratin 10-specific antibody 16855-1-APDownload protocol
IF protocol for Cytokeratin 10-specific antibody 16855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols