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KRI1 Polyclonal antibody

KRI1 Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 16243-1-AP

Print datasheet

Synonyms

KRI1, KRI1 homolog (S. cerevisiae), Protein KRI1 homolog

验证数据展示

  • WB analysis of HEK-293 using 16243-1-AP

    WB analysis of HEK-293 using 16243-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16243-1-AP (KRI1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 16243-1-AP

    IF Staining of HepG2 using 16243-1-AP

    Immunofluorescent analysis of HepG2 cells using 16243-1-AP (KRI1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 16243-1-AP is KRI1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen KRI1 fusion protein Ag9171 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name KRI1 homolog (S. cerevisiae)
Synonyms KRI1, KRI1 homolog (S. cerevisiae), Protein KRI1 homolog
Calculated Molecular Weight 709 aa, 80 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC009876
Gene Symbol KRI1
Gene ID (NCBI) 65095
RRIDAB_2878235
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N9T8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KRI1 antibody 16243-1-APDownload protocol
IF protocol for KRI1 antibody 16243-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols