KRAS-2A-specific Polyclonal antibody
KRAS-2A-specific Polyclonal Antibody for FC, IF/ICC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
FC, IF/ICC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | NIH/3T3 cells |
Positive IF detected in | SH-SY5Y cells |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
产品信息
16156-1-AP targets KRAS-2A-specific in WB, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | FC, IF/ICC, WB, ELISA |
Cited Applications | WB |
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog |
Synonyms | C K RAS, c Ki ras, GTPase KRas, K Ras 2, K RAS2A, K RAS2B, K RAS4A, K RAS4B, KI RAS, KRAS, KRAS1, KRAS2, KRAS2A, KRAS-2A-specific, KRAS2B speicifc, KRAS4A, Krasa, Krasb, NS3, RASK2 |
Calculated Molecular Weight | 21 kDa |
Observed Molecular Weight | 21-24 kDa |
GenBank Accession Number | NM_033360 |
Gene Symbol | KRAS |
Gene ID (NCBI) | 3845 |
RRID | AB_2234477 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P01116 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for KRAS-2A-specific antibody 16156-1-AP | Download protocol |
IF protocol for KRAS-2A-specific antibody 16156-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Chem Biol Targeting Endogenous K-RAS for Degradation through the Affinity-Directed Protein Missile System. | ||
Sci Signal Evaluation of the selectivity and sensitivity of isoform- and mutation-specific RAS antibodies. |